A Reversible Fluorescent Probe for Real-Time Quantitative Monitoring of Cellular Glutathione
- 주제(키워드) antioxidants , fluorescent probes , gluthionine , ratiometric probes , redox homeostasis
- 등재 SCIE, SCOPUS
- 발행기관 Wiley-VCH Verlag
- 발행년도 2017
- 총서유형 Journal
- URI http://www.dcollection.net/handler/ewha/000000145611
- 본문언어 영어
- Published As http://dx.doi.org/10.1002/anie.201702114
초록/요약
The ability to monitor and quantify glutathione (GSH) in live cells is essential in order to gain a detailed understanding of GSH-related pathological events. However, owing to their irreversible response mechanisms, most existing fluorescent GSH probes are not suitable for this purpose. We have developed a ratiometric fluorescent probe (QG-1) for quantitatively monitoring cellular GSH. The probe responds specifically and reversibility to GSH with an ideal dissociation constant (Kd) of 2.59 mm and a fast response time (t1/2=5.82 s). We also demonstrate that QG-1 detection of GSH is feasible in a model protein system. QG-1 was found to have extremely low cytotoxicity and was applied to determine the GSH concentration in live HeLa cells (5.40±0.87 mm). © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
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