SRC activates TAZ for intestinal tumorigenesis and regeneration
- 주제(키워드) APC , Colorectal cancer , Regeneration , SRC , TAZ
- 등재 SCIE, SCOPUS
- 발행기관 Elsevier Ireland Ltd
- 발행년도 2017
- URI http://www.dcollection.net/handler/ewha/000000146933
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.canlet.2017.09.003
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Proto-oncogene tyrosine-protein kinase Src (cSRC) is involved in colorectal cancer (CRC) development and damage-induced intestinal regeneration, although the cellular mechanisms involved are poorly understood. Here, we report that transcriptional coactivator with PDZ binding domain (TAZ) is activated by cSRC, regulating CRC cell proliferation and tumor formation, where cSRC overexpression increases TAZ expression in CRC cells. In contrast, knockdown of cSRC decreases TAZ expression. Additionally, direct phosphorylation of TAZ at Tyr316 by cSRC stimulates nuclear localization and facilitates transcriptional enhancer factor TEF-3 (TEAD4)-mediated transcription. However, a TAZ phosphorylation mutant significantly decreased cell proliferation, wound healing, colony forming, and tumor formation. In a CRC mouse model, ApcMin/+, activated SRC expression was associated with increased TAZ expression in polyps and TAZ depletion decreased polyp formation. Moreover, intestinal TAZ knockout mice had intestinal regeneration defects following γ-irradiation. Finally, significant correspondence between SRC activation and TAZ overexpression was observed in CRC patients. These results suggest that TAZ is a critical factor for SRC-mediated intestinal tumor formation and regeneration. © 2017 Elsevier B.V.
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