Preparation of phytosteryl ester and simultaneous enrichment of stearidonic acid via lipase-catalyzed esterification
- 주제(키워드) Ahiflower™ seed oil , Candida rugosa lipase , Esterification , Phytosterol , Phytosteryl ester , Stearidonic acid
- 등재 SCIE, SCOPUS
- 발행기관 Elsevier Ltd
- 발행년도 2017
- URI http://www.dcollection.net/handler/ewha/000000146951
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.procbio.2017.06.013
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
The simultaneous synthesis of a phytosteryl ester and enrichment of stearidonic acid (SDA) were performed via a one-step lipase-catalyzed esterification of fatty acids from Ahiflower™ seed oil with phytosterol. A commercial lipase (Lipase OF) from Candida rugosa was employed as a biocatalyst. Three solvents were screened and cyclohexane was selected as a suitable reaction medium. The effects of enzyme loading, temperature, and solvent amount were investigated. The conversion as well as the SDA content were significantly improved by adding molecular sieves after 1 h of reaction. The optimum conditions were the enzyme loading of 10% (based on the total substrate weight), the temperature of 30 °C, and the solvent amount of 4 mL (based on a substrate weight of 2 g), respectively. The maximum conversion and SDA content in the residual fatty acid were 81 mol% and 58 mol%, respectively, under the optimum conditions. The SDA content increased a 3.4-fold from 17 mol% in Ahiflower™ seed oil to 58 mol%. © 2017 Elsevier Ltd
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