A Far-Red-Emitting Fluorescence Probe for Sensitive and Selective Detection of Peroxynitrite in Live Cells and Tissues
- 등재 SCIE, SCOPUS
- 발행기관 American Chemical Society
- 발행년도 2017
- URI http://www.dcollection.net/handler/ewha/000000146954
- 본문언어 영어
- Published As http://dx.doi.org/10.1021/acs.analchem.7b02707
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
In this study, the far-red-emitting fluorescence probe 1, containing a rhodamine derivative and a hydrazide reactive group, was developed for peroxynitrite detection and imaging. This probe, which is cell permeable and shows high sensitivity and selectivity in fluorometric detection of peroxynitrite over other ROS/RNS, was successfully utilized to detect exogenous and endogenous peroxynitrite in HeLa and RAW 264.7 cells, respectively. More importantly, 1 can also be used to detect endogenous peroxynitrite generated in Pseudomonas aeruginosa (PAO1)-infected mouse bone marrow-derived neutrophils. We anticipate that the new probe will serve as a powerful molecular imaging tool in investigations of the role(s) played by peroxynitrite in a variety of physiological and pathological contexts. (Figure Presented). © 2017 American Chemical Society.
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