Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process
- 주제(키워드) Corynebacterium glutamicum , Metabolic engineering , 5-Aminovaleric acid , Miscanthus , Pretreatment
- 주제(기타) Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- 발행기관 ELSEVIER SCI LTD
- 발행년도 2017
- URI http://www.dcollection.net/handler/ewha/000000149886
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.biortech.2017.05.131
초록/요약
This study examined nine expired industrial Corynebacterium glutamicum strains with high lysine producing capability for enhanced production of 5-AVA. C. glutamicum KCTC 1857 exhibiting the highest lysine production was transformed with either original Pseudomonas putida davBA genes, encoding the 5-AVA biosynthesis pathway, or C. glutamicum codon-optimized davBA genes. C. glutamicum KCTC 1857 expressing the original genes had superior cell viability and 5-AVA production capability compared to the other strain. This strain produced 39.93 g/L of 5-AVA, which is the highest titer reported to date in fed-batch fermentation from glucose. Indeed, Miscanthus hydrolysate solution prepared from a novel process, comprising pretreatment, hydrolysis, purification, and concentration, was used as feedstock for 5-AVA production. A total of 12.51 g/L 5-AVA was produced from the Miscanthus hydrolysate; this value is 34.7% higher than that obtained from glucose in batch fermentation. (C) 2017 Elsevier Ltd. All rights reserved.
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