Longitudinal Monitoring of Antibody Responses against Tumor Cells Using Magneto-nanosensors with a Nanoliter of Blood
- 주제(키워드) Magneto-nanosensors , bioluminescent imaging , magnetic nanoparticles , lymphoma , cancer immunology , immunoglobulin isotypes
- 주제(기타) Chemistry, Multidisciplinary; Chemistry, Physical; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied; Physics, Condensed Matter
- 설명문(일반) [Lee, Jung-Rok] Ewha Womans Univ, Div Mech & Biomed Engn, Seoul 03760, South Korea; [Lee, Jung-Rok; Wang, Shan X.] Stanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USA; [Chan, Carmel T.; Chuang, Hui-Yen; Mallick, Parag; Gambhir, Sanjiv S.; Wang, Shan X.] Stanford Univ, Dept Med, Dept Radiol, Stanford, CA 94305 USA; [Ruderman, Daniel] USC Keck Sch Med, Ellison Inst Transformat Med USC, Los Angeles, CA 90211 USA; [Gaster, Richard S.] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA; [Gaster, Richard S.] Pliant Therapeut, Redwood City, CA 94063 USA; [Atallah, Michelle] Stanford Sch Med, Canc Biol Program, Stanford, CA 94305 USA; [Lowe, Scott W.] Mem Sloan Kettering Canc Ctr, Canc Biol & Genet Program, New York, NY 10065 USA; [Wang, Shan X.] Stanford Univ, Dept Elect Engn, Stanford, CA 94305 USA
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- 발행기관 AMER CHEMICAL SOC
- 발행년도 2017
- URI http://www.dcollection.net/handler/ewha/000000149956
- 본문언어 영어
- Published As http://dx.doi.org/10.1021/acs.nanolett.7b02591
초록/요약
Each immunoglobulin isotype has unique immune effector functions. The contribution of these functions in the elimination of pathogens and tumors can be determined by monitoring quantitative temporal changes in isotype levels. Here, we developed a novel technique using magneto-nanosensors based on the effect of giant magneto-resistance (GMR) for longitudinal monitoring of total and antigen-specific isotype levels with high precision, using as little as 1 nL of serum. Combining in vitro serologic measurements with in vivo imaging techniques, we investigated the role of the antibody response in the regression of firefly luciferase (FL)-labeled lymphoma cells in spleen, kidney, and lymph nodes in a syngeneic Burkitt's lymphoma mouse model. Regression status was determined by whole body bioluminescent imaging (BLI). The magneto-nanosensors revealed that anti-FL IgG2a and total IgG2a were elevated and sustained in regression mice compared to non-regression mice (p < 0.05). This platform shows promise for monitoring immunotherapy, vaccination, and autoimmunity.
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