One-step fermentative production of aromatic polyesters from glucose by metabolically engineered Escherichia coli strains
- 주제(기타) Multidisciplinary Sciences
- 설명문(일반) [Yang, Jung Eun; Kim, Won Jun; Lee, Sang Yup] Korea Adv Inst Sci & Technol, Metab & Biomol Engn Natl Res Lab, Dept Chem & Biomol Engn,Plus Program BK21, Ctr Syst & Synthet Biotechnol,Inst BioCentury, Daejeon 34141, South Korea; [Park, Si Jae] Ewha Womans Univ, Div Chem Engn & Mat Sci, Seoul 03760, South Korea; [Kim, Hyeong Jun; Kim, Bumjoon J.] Korea Adv Inst Sci & Technol, Polymer & Nano Elect Lab, Dept Chem & Biomol Engn, Inst BioCentury,Plus Program BK21, Daejeon 34141, South Korea; [Lee, Hyuk] Korea Res Inst Chem Technol, Div Drug Discovery Res, Daejeon 34114, South Korea; [Shin, Jihoon] Korea Res Inst Chem Technol, Ctr Bio Based Chem, Green Chem & Engn Div, Daejeon 34114, South Korea; [Lee, Sang Yup] Korea Adv Inst Sci & Technol, BioProc Engn Res Ctr, Daejeon 34141, South Korea; [Lee, Sang Yup] Korea Adv Inst Sci & Technol, BioInformat Res Ctr, Daejeon 34141, South Korea
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- 발행기관 NATURE PUBLISHING GROUP
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000149958
- 본문언어 영어
- Published As http://dx.doi.org/10.1038/s41467-017-02498-w
초록/요약
Aromatic polyesters are widely used plastics currently produced from petroleum. Here we engineer Escherichia coli strains for the production of aromatic polyesters from glucose by one-step fermentation. When the Clostridium difficile isocaprenoyl-CoA: 2-hydroxyisocaproate CoA-transferase (HadA) and evolved polyhydroxyalkanoate (PHA) synthase genes are overexpressed in a D-phenyllactate-producing strain, poly(52.3 mol% 3-hydroxybutyrate (3HB)-co-47.7 mol% D-phenyllactate) can be produced from glucose and sodium 3HB. Also, various poly(3HB-co-D-phenyllactate) polymers having 11.0, 15.8, 20.0, 70.8, and 84.5 mol% of D-phenyllactate are produced from glucose as a sole carbon source by additional expression of Ralstonia eutropha beta-ketothiolase (phaA) and reductase (phaB) genes. Fed-batch culture of this engineered strain produces 13.9 g l(-1) of poly(61.9 mol% 3HB-co-38.1 mol% D-phenyllactate). Furthermore, different aromatic polyesters containing D-mandelate and D-3-hydroxy-3-phenylpropionate are produced from glucose when feeding the corresponding monomers. The engineered bacterial system will be useful for one-step fermentative production of aromatic polyesters from renewable resources.
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