Enhanced intracellular delivery of macromolecules by melittin derivatives mediated cellular uptake
- 주제(키워드) Cell-penetrating peptide , Intracellular delivery , Melittin
- 등재 SCIE, SCOPUS, KCI등재
- 발행기관 Korean Society of Industrial Engineering Chemistry
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000150027
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.jiec.2017.09.039
초록/요약
Various cell-penetrating peptides (CPPs) have been utilized as a promising tool for intracellular delivery of therapeutic macromolecules. In this study, hemolytic membrane-active peptide (Melittin) has been utilized for intracellular uptake of macromolecules by its sequential fragmentation. Among its fragmented derivatives, melittin(1-14) showed highly efficient delivery of proteins into HeLa cells, while maintaining their biological activities. The highest level of intracellular delivery was observed when ß-galactosidase and Cytochrome C were co-delivered with melittin(1-14). These data suggested that melittin(1-14) could be a useful adjuvant for the enhanced intracellular delivery of therapeutic macromolecules. © 2017
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