Optimization of leucrose production by dextransucrase from Streptococcus mutans and its application as an adipogenesis regulator
- 주제(키워드) Leucrose , Sucrose , Dextransucrase , Streptococcus mutans , Adipogenesis
- 주제(기타) Food Science & Technology
- 설명문(일반) [Lee, Daeyeon; Yoo, Sang-Ho] Sejong Univ, Dept Food Sci & Biotechnol, 209 Neungdong Ro, Seoul 05006, South Korea; [Lee, Daeyeon; Yoo, Sang-Ho] Sejong Univ, Carbohydrate Bioprod Res Ctr, 209 Neungdong Ro, Seoul 05006, South Korea; [Lee, Jihye; Kim, Yuri] Ewha Womans Univ, Dept Nutr Sci & Food Management, 52 Ewhayeodae Gil, Seoul 03760, South Korea; [Hong, Moon-Gi; Lee, Byung-Hoo] Gachon Univ, Dept Food Sci & Biotechnol, Seongnam 13120, South Korea; [Kim, Young-Min] Chonnam Natl Univ, Dept Biotechnol, Gwangju 61186, South Korea; [Chang, Pahn-Shick] Seoul Natl Univ, Dept Agr Biotechnol, Ctr Food & Bioconvergence, Seoul 08826, South Korea; [Chang, Pahn-Shick] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 08826, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 ELSEVIER SCIENCE BV
- 발행년도 2017
- URI http://www.dcollection.net/handler/ewha/000000150050
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.jff.2017.10.019
초록/요약
Leucrose is a sucrose isomer which has an alpha-1,5-linkage, and slowly hydrolyzed to glucose and fructose by small intestinal alpha-glucosidases. Leucrose can be produced by an isomerization reaction of dextransucrase on a sucrose substrate. In this study, the recombinant dextransucrase from Streptococcus mutans (SmDS) was applied to optimize the reaction conditions for leucrose production. With a substrate mixture of 0.5 M sucrose + 1.0 M fructose, the greatest yield (ca. 24.5%) of leucrose was obtained by SIDS treatment at 30 degrees C for 120 h. When preadipocyte 3T3-L1 cells were treated with leucrose, this disaccharide inhibited intracellular lipid accumulation in a dose-dependent manner and significantly suppressed mRNA levels of major adipogenic genes, including CCAT/enhancer-binding protein alpha (C/EBP alpha), peroxisome proliferator-activated receptor-gamma (PPAR gamma), fatty acid synthase (FAS), and sterol regulatory element-binding protein-1C (SREBP-1C). Phosphorylation of P13 kinase/Akt/mTOR was also reduced with leucrose treatment. These results suggest that leucrose has a potential in regulating adipogenesis.
more