Portable cyanobacteria detection fluorometer with current-conveyor transimpedance amplifiers
- 주제(키워드) Current-conveyor , Cyanobacteria , Fluorometer , Quantum dots , Transimpedance amplifier
- 등재 SCIE, SCOPUS, KCI등재
- 발행기관 Institute of Electronics Engineers of Korea
- 발행년도 2017
- URI http://www.dcollection.net/handler/ewha/000000150109
- 본문언어 영어
- Published As http://dx.doi.org/10.5573/JSTS.2017.17.6.886
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
A portable fluorometer has been developed for cyanobacteria detection by exploiting commercially available low-cost photodiodes. Particularly, the front-end detection circuit was integrated on-chip by using a 0.11-mm CMOS process to realize the high transimpedance gain of 99.7 dBΩ with low current consumption of 1.4 mA from a single 1.5-V supply, hence providing a low-power solution with small form-factor in the applications of portable cyanobacteria detection. For measuring fluorescence intensity of cyanobacteria, two quantum dots of QD565 and QD655 were employed into a NanoGene assay for mcyD gene(synthetase of toxic microcystin) detection of Microcystis aeruginosa, resulting in two different readout voltages. Various operational parameters (e.g. distance, angle between laser emission and vial sample, etc) were investigated for the proposed fluorometer. Measurements demonstrated that the mcyD gene hybridized with QD565 and QD655 was quantitatively detected, i.e. the normalized output voltage (QD655/QD565) was linearly proportional to the mcyD gene copy numbers (y = 0.25 log x – 0.25). Besides, the wireless transfer of the quantitative data was performed via Bluetooth. © 2017, Institute of Electronics Engineers of Korea. All rights reserved.
more