A new kind of rhodamine-based fluorescence turn-on probe for monitoring ATP in mitochondria
- 주제(키워드) ATP sensor , Colorimetric chemosensor , Fluorescence sensor , Mitochondria targeting , Rhodamine
- 등재 SCIE, SCOPUS
- 발행기관 Elsevier B.V.
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000151527
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.snb.2018.03.081
초록/요약
We have developed a new kind of colorimetric and fluorescence “turn-on” probe based on rhodamine derivatives for the detection of adenosine-5′-triphosphate (ATP) through hydrogen bond interactions. Upon the addition of 5 mM ATP, the probe 1 shows a 65-fold enhancement in fluorescence intensity and a color change from colorless to pink. The probe 2 also shows very obvious color and fluorescence change with the presence of ATP. The experimental results show that this kind of probe has high specificity towards ATP over other anions, metal ions, and other nucleoside polyphosphates, except ADP. The enhanced emission and naked eye changes are attributed to spirolactam ring-opening, which is pH independent (pH (4.0–7.4)). The more important finding from our results is that the electron withdrawing group in the chain decreases the binding ability to ATP. The live cell imaging experiments in Hela cells indicated that probe 2 has cell permeability, and mainly locates to mitochondria. © 2018 Elsevier B.V.
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