Near-infrared fluorescent probes for the detection of glutathione and their application in the fluorescence imaging of living cells and tumor-bearing mice
- 주제(기타) Materials Science, Biomaterials
- 설명문(일반) [Lee, Dayoung; Chen, Xiaoqiang; Yoon, Juyoung] Ewha Womans Univ, Dept Chem & Nano Sci BK21, Seoul 120750, South Korea; [Jeong, Keunsoo; Kim, Gayoung; Kim, Sehoon] Korea Inst Sci & Technol, Ctr Theragnosis, Hwarangno 14 Gil 5, Seoul 136791, South Korea; [Luo, Xiao; Yang, Youjun] East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Meilong Rd 130, Shanghai 200236, Peoples R China; [Chen, Xiaoqiang] Nanjing Tech Univ, Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Coll Chem Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 210009, Jiangsu, Peoples R China
- 발행기관 ROYAL SOC CHEMISTRY
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000151530
- 본문언어 영어
- Published As http://dx.doi.org/10.1039/c7tb01560g
초록/요약
Two new cyanine-based fluorescent probes 1 and 2 have been developed. Probe 1 bears two cyanine units in a single molecule, and probe 2 contains a bis(trifluoromethyl)benzenethiol moiety. Both are non-fluorescent. The addition of intracellular glutathione (GSH) significantly enhanced the NIR fluorescence of the two probes. Both probes were used to image varying amounts of GSH in living cells. In tumor bearing mice, the in vivo fluorescence intensity of both probes was higher in tumors, where GSH is overexpressed, than in normal tissues. These results suggest that these new fluorogenic probes have potential for GSH-targeting diagnostic imaging.
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