Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols
- 주제(기타) Chemistry, Multidisciplinary
- 설명문(일반) [Sedgwick, Adam C.; Gardiner, Jordan E.; Jenkins, A. Toby A.; Bull, Steven D.; James, Tony D.] Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England; [Kim, Gyoungmi; Yoon, Juyoung] Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea; [Yevglevskis, Maksims; Lloyd, Matthew D.] Univ Bath, Dept Pharm & Pharmacol, Drug & Target Dev, Bath BA2 7AY, Avon, England
- 등재 SCIE, SCOPUS
- 발행기관 ROYAL SOC CHEMISTRY
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000151531
- 본문언어 영어
- Published As http://dx.doi.org/10.1039/c8cc01661e
초록/요약
Two 'turn on' TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 mu M limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity of TCF-GSH decreased and toxicity was observed for TCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 mu M limit of detection. No toxicity was found for TCFCl-GSH and a clear 'turn on' with good photostability was observed for the exogenous addition of GSH, Cys and HCys. Furthermore, TCFCl-GSH was used to evaluate the effects of drug treatment on the levels of GSH in live cells.
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