A Visible and Near-Infrared, Dual-Channel Fluorescence-On Probe for Selectively Tracking Mitochondrial Glutathione
- 주제(기타) Chemistry, Multidisciplinary
- 설명문(일반) [Xu, Zhiqiang; Han, Xie; Wu, Di; Cao, Meijiao; Liu, Sheng Hua; Yin, Jun] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Hubei, Peoples R China; [Huang, Xiaoting; Zhang, Bibo; Tan, Ying] Tsinghua Univ, Grad Sch Shenzhen, Key Lab Chem Biol, State Key Lab Chem Oncogen, Shenzhen 518055, Guangdong, Peoples R China; [Yoon, Juyoung] Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea
- 등재 SCOPUS
- 발행기관 CELL PRESS
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000151533
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.chempr.2018.04.003
초록/요약
Glutathione is a major endogenous antioxidant in biological systems. As a result, highly selective fluorescent probes are significant components of studies aimed at gaining a better understanding of the role(s) glutathione plays in biological functions. In this study, we developed a dual-channel turn-on fluorescent probe for glutathione, comprising naphthalimide and cyanine, bridged by a thiol-reactive sulfonamide moiety. The probe selectively responds to glutathione and is not affected by cysteine and homocysteine. Important information has been gained about the chemical mechanism for operation of the probe, which responds to glutathione in visible and near-infrared channels synchronously and independently. Moreover, this probe exhibits the remarkable capability of targeting mitochondria and dual-channel tracking of intracellular glutathione in a spatiotemporal and synchronous manner. We believe that the strategy used to design the new dual-channel fluorescence-on probe will be applicable to the design of fluorescent probes that display specific responses in multiple channels.
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