Increased Thrombogenicity in Chronic Renal Failure in a Rat Model Induced by 5/6 Ablation/Infarction
- 주제(키워드) Chronic renal failure , thrombosis , ferric chloride , thromboelastometry
- 주제(기타) Medicine, General & Internal
- 설명문(일반) [Song, Tae-Jin; Lee, Jee-Eun; Han, Kyeo-Rye; Chang, Yoonkyung; Kim, Yong-Jae] Ewha Womans Univ, Coll Med, Dept Neurol, Seoul, South Korea; [Piao, Honglim; Han, Ki-Hwan] Ewha Womans Univ, Coll Med, Dept Anat, Seoul, South Korea; [Kwon, Il; Choi, Hyun-Jung; Lee, Kyung-Yul; Heo, Ji Hoe] Yonsei Univ, Coll Med, Dept Neurol, 50-1 Yonsei Ro, Seoul 03722, South Korea; [Oh, Hyung Jung] Ewha Womans Univ, Ewha Inst Convergence Med, Seoul, South Korea
- 발행기관 YONSEI UNIV COLL MEDICINE
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000151583
- 본문언어 영어
- Published As http://dx.doi.org/10.3349/ymj.2018.59.6.754
초록/요약
Purpose: Abnormalities in hemostasis and coagulation have been suggested in chronic renal failure (C:RF). In this study, we compared processes of thrombus formation between rats with CRF and those with normal kidney function. Materials and Methods: CRF was induced by 5/6 ablation/infarction of the kidneys in Sprague-Dawley rats, and surviving rats after 4 weeks were used. Ferric chloride (FeCl3)-induced thrombosis in the carotid artery was induced to assess thrombus formation. Whole blood dot formation was evaluated using rotational thromboelastometry (ROTEM). Platelet aggregation was assessed with impedance platelet aggregometry. Results: FeCl3-induced thrombus formation was initiated faster in the CRF group than in the control group (13.2 +/- 1.1 sec vs. 17.8 +/- 1.0 sec, p=0.027). On histological examination, the maximal diameters of thrombi were larger in the CRF group than in the control group (394.2 +/- 201.1 pm vs. 114.0 +/- 145.1 pm, p=0.039). In extrinsic pathway ROTEM, the CRF group showed faster dot initiation (dotting time, 59.0 +/- 7.3 sec vs. 72.8 +/- 5.0 sec, p=0.032) and increased dot growth kinetics (a angle, 84.8 +/- 0.2 degrees vs. 82.0 +/- 0.6 degrees, p=0.008), compared to the control group. Maximal platelet aggregation rate was higher in the CRF group than in the control group (58.2 +/- 0.2% vs. 44.6 +/- 1.2%, p=0.006). Conclusion: Our study demonstrated that thrombogenicity is increased in rats with CRF. An activated extrinsic coagulation pathway may play an important role in increasing thrombogenicity in CRF.
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