Effect of apoptosis-associated speck-like protein containing a caspase recruitment domain on vaccine efficacy: Overcoming the effects of its deficiency with aluminum hydroxide adjuvant
- 주제(키워드) alum , ASC , HPV vaccine , influenza vaccine
- 주제(기타) Immunology; Microbiology
- 설명문(일반) [Lee, Deuk-Ki; Lee, Eun-Young; Kim, Ryoon-Ho; Kwak, Hye-Won; Nam, Jae-Hwan] Catholic Univ Korea, Dept Biotechnol, 43-1 Yeokgok 2 Dong, Bucheon 14662, Gyeonggi Do, South Korea; [Kim, Joo Young; Chang, Jun] Ewha Womans Univ, Div Life & Pharmaceut Sci, Seoul 03760, South Korea; [Kim, Hun] SK Chem, Seongnam Si 13493, Gyeonggi Do, South Korea; [Kang, Kyung-Won; Lee, Sang-Myeong] Chonbuk Natl Univ, Dept Biotechnol, Iksan 570752, South Korea; [Park, Jong-Hwan] Chonnam Natl Univ, Coll Vet Med, Lab Anim Med, Gwangju 61186, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 WILEY
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000151611
- 본문언어 영어
- Published As http://dx.doi.org/10.1111/1348-0421.12569
초록/요약
Host factors such as nutritional status and immune cell state are important for vaccine efficacy. Inflammasome activation may be important for triggering vaccine-induced humoral and cell-mediated immune responses. Formulations with alum as a typical adjuvant to overcome the effects of host factors have recently been shown to induce inflammasome activation, which augments vaccine efficacy. Apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) is one of the main components of inflammasomes, but it is not clear whether ASC affects the vaccine-induced immune response. Herein, we used two types of vaccines: inactivated influenza vaccine not formulated with alum, and HPV vaccine formulated with alum. We gave the vaccines to ASC knockout (ASC(-/-)) mice to investigate the role of ASC in vaccine efficacy. Influenza vaccine-immunized ASC(-/-) mice did not show antibody titers in week 2 after the first vaccination. After boosting, the antibody titer in ASC(-/-) mice was about half that in wild type (WT) mice. Furthermore, a cytotoxic T-lymphocyte response against influenza vaccine was not induced in ASC(-/-) mice. Therefore, vaccinated ASC(-/-) mice did not show effective protection against viral challenge. ASC(-/-) mice immunized with alum-formulated HPV vaccine showed similar antibody titers and T-cell proliferation compared with immunized WT mice. However, the HPV vaccine without alum induced up to threefold lower titers of HPV-specific antibody titers in ASC(-/-) mice compared with those in WT mice. These findings suggest that alum in vaccine can overcome the ASC-deficient condition.
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