Sustained release of parathyroid hormone via in situ cross-linking gelatin hydrogels improves the therapeutic potential of tonsil-derived mesenchymal stem cells for hypoparathyroidism
- 주제(키워드) parathyroid hormone , gelatin hydrogels , tonsil-derived mesenchymal stem cells , ionized calcium , hypoparathyroidism , parathyroidectomized animal
- 주제(기타) Cell & Tissue Engineering; Biotechnology & Applied Microbiology; Cell Biology; Engineering, Biomedical
- 설명문(일반) [Park, Yoon Shin; Jin, Yoon Mi; Kim, Gyungah; Jo, Inho] Ewha Womans Univ, Sch Med, Dept Mol Med, Seoul 07985, South Korea; [Park, Yoon Shin; Jin, Yoon Mi; Kim, Gyungah; Jung, Sung Chul; Jo, Inho] Ewha Womans Univ, Sch Med, Ewha Tonsil Derived Mesenchymal Stem Cells Res Ct, Seoul, South Korea; [Park, Yoon Shin] Chungbuk Natl Univ, Sch Biol Sci, Coll Nat Sci, Cheongju, South Korea; [Lee, Yunki; Park, Ki Dong] Ajou Univ, Dept Mol Sci & Technol, San 5,Woncheon Dong, Suwon 16499, South Korea; [Jung, Sung Chul] Ewha Womans Univ, Sch Med, Dept Biochem, Seoul, South Korea; [Park, Yoon Jeong] Seoul Natl Univ, Dent Res Inst, Sch Dent, Dept Dent Regenerat Biotechnol, Seoul, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 WILEY
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000151627
- 본문언어 영어
- Published As http://dx.doi.org/10.1002/term.2430
초록/요약
Biomimetic parathyroid regeneration with sustained release of parathyroid hormone (PTH) into the blood stream is a considerable challenge in hypoparathyroidism treatment. We recently reported that tonsil-derived mesenchymal stem cells (TMSCs), if these cells were both differentiated in vitro before implantation and incorporated into a scaffold Matrigel, are a good cell source for parathyroid regeneration in a parathyroidectomized (PTX) animal model. Here, we present a new strategy for improved clinical application that enhances the sustained release of PTH by controlling mechanical stiffness using in situ-forming gelatin-hydroxyphenyl propionic acid (GH) hydrogels (GHH). Differentiated TMSCs (dTMSCs) embedded in a GHH with a strength of 4.4kPa exhibited the best sustained release of PTH and were the most effective in hypoparathyroidism treatment, showing improved blood calcium homeostasis compared with Matrigel-embedded dTMSCs. Interestingly, undifferentiated control TMSCs (cTMSCs) also released PTH in a sustained manner if incorporated into GHH. Collectively, these findings may establish a new paradigm for parathyroid regeneration that could ultimately evolve into an improved clinical application. Copyright (c) 2017 John Wiley & Sons, Ltd.
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