Effect of vitamin C on azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced colitis-associated early colon cancer in mice
- 주제(키워드) Vitamin C , colitis , inflammation , colonic neoplasm , microbiota
- 주제(기타) Nutrition & Dietetics
- 설명문(일반) [Jeon, Hee-Jin; Yeom, Yiseul; Kim, Yoo-Sun; Kim, Eunju; Kim, Yuri] Ewha Womans Univ, Dept Nutr Sci & Food Management, 52 Ewhayeodae Gil, Seoul 03760, South Korea; [Shin, Jae-Ho; Seok, Pu Reum] Eulji Univ, Dept Biomed Lab Sci, Gyeonggi 13135, South Korea; [Woo, Moon Jea] Kwang Dong Pharmaceut Co Ltd, Seoul 06650, South Korea
- 등재 SCIE, SCOPUS, KCI등재
- 발행기관 KOREAN NUTRITION SOC
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000151711
- 본문언어 영어
- Published As http://dx.doi.org/10.4162/nrp.2018.12.2.101
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
BACKGROUD/OBJECTIVES: The objective of this study was to investigate the effects of vitamin C on inflammation, tumor development, and dysbiosis of intestinal microbiota in an azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced inflammation-associated early colon cancer mouse model.& para;& para;MATERIALS/METHODS: Male BALB/c mice were injected intraperitoneally with AOM [10 mg/kg body weight (b.w)] and given two 7-d cycles of 2% DSS drinking water with a 14 d inter-cycle interval. Vitamin C (60 mg/kg b.w. and 120 mg/kg b.w.) was supplemented by gavage for 5 weeks starting 2 d after the AOM injection.& para;& para;RESULTS: The vitamin C treatment suppressed inflammatory morbidity, as reflected by disease activity index (DAI) in recovery phase and inhibited shortening of the colon, and reduced histological damage. In addition, vitamin C supplementation suppressed mRNA levels of pro-inflammatory mediators and cytokines, including cyclooxygenase-2, microsomal prostaglandin E synthase-2, tumor necrosis factor-alpha, Interleukin (11.)-1 beta, and IL-6, and reduced expression of the proliferation marker, proliferating cell nuclear antigen, compared to observations of AOM/DSS animals. Although the microbial composition did not differ significantly between the groups, administration of vitamin C improved the level of inflammation-related Lactococcus and JQ084893 to control levels.& para;& para;CONCLUSION: Vitamin C treatment provided moderate suppression of inflammation, proliferation, and certain inflammation-related dysbiosis in a murine model of colitis associated-early colon cancer. These findings support that vitamin C supplementation can benefit colonic health. Long-term clinical studies with various doses of vitamin C are warranted.
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