Cellular uptake mechanism and comparative in vitro cytotoxicity studies of monomeric LMWP-siRNA conjugate
- 주제(키워드) Cell penetrating peptide , Cell uptake mechanism , Covalent CPP-siRNA chemical conjugate , Cytotoxicity , siRNA delivery
- 등재 SCIE, SCOPUS, KCI등재
- 발행기관 Korean Society of Industrial Engineering Chemistry
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000151754
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.jiec.2018.02.005
초록/요약
The covalent attachment of CPPs to siRNA molecules offers great potential for CPP-mediated siRNA delivery. We recently reported a concise and high-yield synthesis strategy of the cell-permeable, cytosol-dissociable LMWP-siRNA covalent conjugate. Herein, cell uptake mechanism and cellular toxicity studies of this conjugate were performed to evaluate the potential of LMWP-siRNA conjugate for clinical translation. Cellular uptake mechanism study indicated that the conjugate could be taken up by cells via multiple pathways, including direct penetration of the plasma membrane and clathrin- and caveolae-independent endocytosis. In vitro cytotoxicity study revealed that the conjugation promoted internalization in a low-toxic fashion. © 2018 The Korean Society of Industrial and Engineering Chemistry
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