D-tyrosine negatively regulates melanin synthesis by competitively inhibiting tyrosinase activity
- 주제(키워드) D-tyrosine , L-tyrosine , melanin , melanogenesis , tyrosinase
- 주제(기타) Oncology; Cell Biology; Dermatology
- 설명문(일반) [Park, Jisu; Oh, Eok-Soo] Ewha Womans Univ, Res Ctr Cellular Homeostasis, Dept Life Sci, Seoul, South Korea; [Jung, Hyejung; Oh, Eok-Soo] Skin QC Inst Dermatol Sci, Seoul, South Korea; [Kim, Kyuri; Lim, Kyung-Min] Ewha Womans Univ, Coll Pharm, Seoul, South Korea; [Kim, Ji-young; Jho, Eek-hoon] Univ Seoul, Dept Life Sci, Seoul, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 WILEY
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000151776
- 본문언어 영어
- Published As http://dx.doi.org/10.1111/pcmr.12668
초록/요약
Although L-tyrosine is well known for its melanogenic effect, the contribution of D-tyrosine to melanin synthesis was previously unexplored. Here, we reveal that, unlike L-tyrosine, D-tyrosine dose-dependently reduced the melanin contents of human MNT-1 melanoma cells and primary human melanocytes. In addition, 500 mu M of D-tyrosine completely inhibited 10 mu M L-tyrosine-induced melanogenesis, and both in vitro assays and L-DOPA staining MNT-1 cells showed that tyrosinase activity is reduced by D-tyrosine treatment. Thus, D-tyrosine appears to inhibit L-tyrosine-mediated melanogenesis by competitively inhibiting tyrosinase activity. Furthermore, we found that D-tyrosine inhibited melanogenesis induced by alpha-MSH treatment or UV irradiation, which are the most common environmental factors responsible for melanin synthesis. Finally, we confirmed that D-tyrosine reduced melanin synthesis in the epidermal basal layer of a 3D human skin model. Taken together, these data suggest that D-tyrosine negatively regulates melanin synthesis by inhibiting tyrosinase activity in melanocyte-derived cells.
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