Leptin regulates the pro-inflammatory response in human epidermal keratinocytes
- 주제(키워드) Leptin , Epidermal keratinocytes , Pro-inflammatory response , Centromere proteins , Gene ontology enrichment analysis
- 주제(기타) Dermatology
- 설명문(일반) [Lee, Moonyoung; Lee, Eunyoung; Jin, Sun Hee; Ahn, Sungjin; Kim, Sae On; Kim, Jungmin; Noh, Minsoo] Seoul Natl Univ, Coll Pharm, Seoul, South Korea; [Lee, Moonyoung; Lee, Eunyoung; Jin, Sun Hee; Ahn, Sungjin; Kim, Sae On; Kim, Jungmin; Noh, Minsoo] Seoul Natl Univ, Coll Pharm, Inst Nat Prod Res, 1 Gwanak Ro, Seoul 08826, South Korea; [Choi, Dalwoong] Korea Univ, Dept Publ Hlth Sci, Grad Sch, Seoul 02841, South Korea; [Choi, Dalwoong] Korea Univ, Coll Publ Hlth Sci, Seoul 02841, South Korea; [Lim, Kyung-Min] Ewha Womans Univ, Coll Pharm, Seoul 03760, South Korea; [Lee, Seung-Taek] Yonsei Univ, Dept Biochem, Coll Life Sci & Biotechnol, Seoul 03722, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 SPRINGER
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000151778
- 본문언어 영어
- Published As http://dx.doi.org/10.1007/s00403-018-1821-0
초록/요약
The role of leptin in cutaneous wound healing process has been suggested in genetically obese mouse studies. However, the molecular and cellular effects of leptin on human epidermal keratinocytes are still unclear. In this study, the whole-genome-scale microarray analysis was performed to elucidate the effect of leptin on epidermal keratinocyte functions. In the leptin-treated normal human keratinocytes (NHKs), we identified the 151 upregulated and 53 downregulated differentially expressed genes (DEGs). The gene ontology (GO) enrichment analysis with the leptin-induced DEGs suggests that leptin regulates NHKs to promote pro-inflammatory responses, extracellular matrix organization, and angiogenesis. Among the DEGs, the protein expression of IL-8, MMP-1, fibronectin, and S100A7, which play roles in which is important in the regulation of cutaneous inflammation, was confirmed in the leptin-treated NHKs. The upregulation of the leptin-induced proteins is mainly regulated by the STAT3 signaling pathway in NHKs. Among the downregulated DEGs, the protein expression of nucleosome assembly-associated centromere protein A (CENPA) and CENPM was confirmed in the leptin-treated NHKs. However, the expression of CENPA and CENPM was not coupled with those of other chromosome passenger complex like Aurora A kinase, INCENP, and survivin. In cell growth kinetics analysis, leptin had no significant effect on the cell growth curves of NHKs in the normal growth factor-enriched condition. Therefore, leptin-dependent downregulation of CENPA and CENPM in NHKs may not be directly associated with mitotic regulation during inflammation.
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