Metabolic Engineering of Corynebacterium glutamicum for the High-Level Production of Cadaverine That Can Be Used for the Synthesis of Biopolyamide 510
- 주제(키워드) Biopolyamide , PA510 , Cadaverine , L-Lysine , Recombinant Corynebacterium glutamicum , Lysine decarboxylase
- 주제(기타) Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical
- 설명문(일반) [Kim, Hee Taek; Oh, Young Hoon; Hyun, Sung Min; Khang, Tae Uk; Kang, Kyoung Hee; Song, Bong Keun; Joe, Jeong Chan] Korea Res Inst Chem Technol, Div Convergence Chem, Ctr Biobased Chem, POB 107,141 Gajeong Ro, Daejeon 34114, South Korea; [Baritugo, Kei-Anne; Jung, Sol Hee; Park, Si Jae] Ewha Womans Univ, Div Chem Engn & Mat Sci, 52 Ewhayeodae Gil, Seoul 03760, South Korea; [Hyun, Sung Min; Park, Kyungmoon] Hongik Univ, Dept Biol & Chem Engn, 2639 Sejong Ro, Sejong Si 30016, South Korea; [Khang, Tae Uk] Korea Univ, Dept Chem & Biol Engn, 145 Anam Ro, Seoul 02841, South Korea; [Kim, Il-Kwon] DAESANG Corp, Bioproc R&D Ctr, Icheon Si 17384, Gyeonggi Do, South Korea; [Lee, Myung Ock; Kam, Yeji; Hwang, Yong Taek] Lotte Chem, 115 Gajeongbuk Ro, Daejeon 34110, South Korea; [Lee, Myung Ock] Korea Adv Inst Sci & Technol, Dept Chem, 291 Daehak Ro, Daejeon 34141, South Korea
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- 발행기관 AMER CHEMICAL SOC
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000151893
- 본문언어 영어
- Published As http://dx.doi.org/10.1021/acssuschemeng.8b00009
초록/요약
Fermentative production of cadaverine from renewable resources may support a sustainable biorefinery process to produce carbon-neutral nylons such as biopolyamide 510 (PA.510). Cost-competitive production of cadaverine is a key factor in the successful commercialization of PA510. In this study, an integrated biological and chemical process involving cadaverine biosynthesis, purification, and its polymerization with sebacic acid was developed to produce bio-PA510. To stably express ldcC from Escherichia coli in an engineered Corynebacterium glutamicum PKC strain, an expired industrial L-lysine-producing strain, ldcC, was integrated into the chromosome of the C. glutamicum PKC strain by disrupting lysE and controlling its expression via a strong synthetic H30 promoter. Cadaverine was produced at a concentration of 103.78 g/L, the highest titer to date, from glucose by fed-batch culture of this engineered C. glutamgicum PKC strain. Fermentation-derived cadaverine was purified to polymer-grade biocadaverine with high purity (99%) by solvent extraction with chloroform and two-step distillation. Finally, biobased PAS10 with good thermal properties (T-m 215 degrees C and T-c 158 degrees C) was produced by polymerization of purified cadaverine with sebacic acid. The hybrid biorefinery process combining biological and chemical processes demonstrated in this study is a useful platform for producing biobased chemicals and polymers.
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