Microbiome Profiles of Commercial Broilers Through Evisceration and Immersion Chilling During Poultry Slaughter and the Identification of Potential Indicator Microorganisms
- 주제(키워드) poultry , Salmonella , Pseudomonas , microbiome , next generation sequencing , slaughter
- 주제(기타) Microbiology
- 설명문(일반) [Handley, John A.; Park, Si Hong; Kim, Sun Ae; Ricke, Steven C.] Univ Arkansas, Dept Food Sci, Ctr Food Safety, Fayetteville, AR 72703 USA; [Park, Si Hong] Oregon State Univ, Dept Food Sci & Technol, Corvallis, OR 97331 USA; [Kim, Sun Ae] Ewha Womans Univ, Dept Food Sci & Engn, Seoul, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 FRONTIERS MEDIA SA
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000151912
- 본문언어 영어
- Published As http://dx.doi.org/10.3389/fmicb.2018.00345
초록/요약
Commercial poultry abattoirs were evaluated to determine the efficacy of the multi-hurdle antimicrobial strategy employed to reduce the microbial load present on incoming broilers from the farm. As next generation sequencing (NGS) has been recently employed to characterize the poultry production system, this study utilized 16S High throughput sequencing (HTS) and quantitative plating data to profile the microbiota of chicken carcasses and determine the efficacy of the multi-hurdle antimicrobial system. Aerobic plate count (APC) and Enterobacteriaceae (EB) microbial counts were quantified from whole bird carcass rinsates (WBCR). The remaining rinsates underwent microbiome analysis using 16S rRNA gene fragments on an Illumina MiSeq and were analyzed by Quantitative Insights into Microbial Ecology (QIIME). The key stages of processing were determined to be at rehang, pre-chill, and post-chill as per the Salmonella Reduction Regulation (75 Fed. Reg. 27288-27294). The APC microbial data from rehang, pre-chill, and post-chill were mean log 4.63 CFU/mL, 3.21 CFU/mL, and 0.89 CFU/mL and EB counts were mean log 2.99 CFU/mL, 1.95 CFU/mL, and 0.35 CFU/mL. NGS of WBCR identified 222 Operational Taxonomic Units' (OTU's) of which only 23 OTU's or 10% of the population was recovered post-chill. Microbiome data suggested a high relative abundance of Pseudomonas at post-chill. Additionally, Pseudomonas, Enterobacteriaceae, and Weeksellaceae Chryseobacterium have been identified as potential indicator organisms having been isolated from all processing abattoirs and sampling locations. This study provides insight into the microbiota of commercial broilers during poultry processing.
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