Endoplasmic Reticulum-Targeted Ratiometric N-Heterocyclic Carbene Borane Probe for Two-Photon Microscopic Imaging of Hypochlorous Acid
- 주제(기타) Chemistry, Analytical
- 설명문(일반) [Pak, Yen Leng; Yim, Yubin; Boufard, Jean; Yoon, Juyoung] Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 03760, South Korea; [Park, Sang Jun; Kim, Hwan Myung] Ajou Univ, Dept Energy Syst Res, Suwon 443749, Gyeonggi Do, South Korea; [Song, Gyeongok; Kang, Hyuk; Kim, Hwan Myung] Ajou Univ, Dept Chem, Suwon 443749, Gyeonggi Do, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 AMER CHEMICAL SOC
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000155985
- 본문언어 영어
- Published As http://dx.doi.org/10.1021/acs.analchem.8b03565
초록/요약
The naphthoimidazolium borane 4 is shown to be a selective probe for HOCl over other reactive oxygen species. Unlike other boronate-reactive oxygen species (ROS) fluorogenic probes that are oxidized by HOCl through a nucleophilic borono-Dakin oxidation mechanism, probe 4 is distinguished by its electrophilic oxidation mechanism involving B-H bond cleavage. Two-photon microscopy experiments in living cells and tissues with the probe 4 demonstrate the monitoring of endogenous HOCl generation and changes in HOCl concentrations generated in the endoplasmic reticulum during oxidative stress situations.
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