Single mucosal vaccination targeting nucleoprotein provides broad protection against two lineages of influenza B virus
- 주제(키워드) Influenza B virus , Mucosal immunization , Nucleoprotein , Protection , Vaccine
- 등재 SCIE, SCOPUS
- 발행기관 Elsevier B.V.
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000156207
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.antiviral.2019.01.002
- PubMed https://pubmed.ncbi.nlm.nih.gov/30639307
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Nucleoprotein is highly conserved among each type of influenza viruses (A and B) and has received significant attention as a good target for universal influenza vaccine. In this study, we determined whether a recombinant adenovirus encoding nucleoprotein of type B influenza virus (rAd/B-NP) confers protection against influenza virus infection in mice. We also identified a cytotoxic T lymphocyte epitope in the nucleoprotein to determine B-NP-specific CD8 T-cell responses. We found that B-NP-specific CD8 T cells induced by rAd/B-NP immunization played a major role in protection following influenza B virus infection using CD8 knockout mice. To assess the effects of the administration routes on protective immunity, we immunized mice with rAd/B-NP via intranasal or intramuscular routes. Both groups showed strong NP-specific humoral and CD8 T-cell responses, but only intranasal immunization provided complete protection against both lineages of influenza B virus challenge. Intranasal but not intramuscular administration established resident memory CD8 T cells in the airway and lung parenchyma, which were required for efficient protection. Furthermore, rAd/B-NP in combination with rAd/A-NP protected mice against lethal infection with both influenza A and B viruses. These findings demonstrate that rAd/B-NP could be further developed as a universal vaccine against influenza. © 2019 Elsevier B.V.
more