Ultrasensitive colocalization detection based on plasmonic nanolithography with molecular-overlapped optical near-fields
- 주제(키워드) biosensor , colocalization , plasmon lithography , Surface plasmon resonance
- 등재 SCOPUS
- 발행기관 SPIE
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000156218
- ISBN 9781510614970
- 본문언어 영어
- Published As http://dx.doi.org/10.1117/12.2291449
초록/요약
The detection sensitivity of surface plasmon resonance (SPR) biosensors has been improved by employing colocalization of spatial distribution of electromagnetic near-fields and detection molecules. We have used plasmon nanolithography to achieve light-matter colocalization on triangular nanoaperture arrays and optimized array configurations to improve colocalization efficiency. Streptavidin-biotin interactions were measured to validate the concept. It was confirmed that colocalized distributions of target binding and localized near-fields produced larger optical detection sensitivity. The colocalized detection was also shown to come with wider dynamic range than noncolocalized detection. The effective limit-of-detection of colocalized measurements was on the order of 30 pM. The colocalized detection sensitivity was estimated to be below 1 fg/mm2 in a 100-nm deep evanescent area, an enhancement by more than three orders of magnitude over conventional SPR sensor. © 2018 SPIE.
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