Intracellular transformation rates of fatty acids are influenced by expression of the fatty acid transporter FadL in Escherichia coli cell membrane
- 주제(키워드) Long-chain fatty acid transporter FadL , Fatty acids , Whole cell biotransformation , Escherichia coli
- 주제(기타) Biotechnology & Applied Microbiology
- 설명문(일반) [Jeon, Eun-Yeong; Song, Ji-Won; Cha, Hee-Jeong; Park, Jin-Byung] Ewha Womans Univ, Dept Food Sci & Engn, Seoul 03760, South Korea; [Lee, Sun-Mee] Daejeon Univ, Dept Food Sci & Nutr, Daejeon 34520, South Korea; [Lee, Jinwon] Sogang Univ, Dept Chem & Biomol Engn, Seoul 04107, South Korea; [Park, Jin-Byung] Ewha Womans Univ, Inst Mol Microbiol & Biosyst Engn, Seoul 03760, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 ELSEVIER SCIENCE BV
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000156237
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.jbiotec.2018.07.019
초록/요약
Fatty acids have a low permeability through the cell membrane. Therefore, the intracellular biotransformation of fatty acids can be slow due to supply limitations. The effects of expression level of the fatty acid transporter FadL in Escherichia coli on the biotransformations were investigated. The enhanced expression of FadL led to 5.5-fold increase of the maximum reaction rate V-max (i.e., 200 mu mol/min per g dry cells (200 U/g dry cells)) of the recombinant E. coli expressing a hydratase of Stenotrophomonas maltophilia in the periplasm with respect to hydration of oleic acid. The FadL expression level was also critical for oxidation of 12- and 10-hydroxyoctadecanoic acid by the recombinant E. coli expressing an alcohol dehydrogenase (ADH) of Micrococcus luteus. In addition, the multistep biotransformation of ricinoleic acid into the ester (i.e., (Z)-11-(heptanoyloxy) undec-9-enoic acid) by the recombinant E. coli expressing the ADH of M. luteus and a Baeyer-Villiger monooxygenase of Pseudomonas putida KT2440 was 2-fold increased to 40 U/g dry cells with expression of FadL to an appropriate level. The FadL expression level is one of the critical factors to determine whole-cell biotransformation rates of not only long chain fatty acids but also hydroxy fatty acids. This study may contribute to whole-cell biocatalyst engineering for biotransformation of hydrophobic substances.
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