Construction of a scFv library with synthetic, non-combinatorial CDR diversity
- 주제(키워드) Antibody library , Non-combinatorial CDR diversity , Phage display , scFv , Synthetic antibody library , Synthetic CDR diversity
- 후원정보 Ministry of Science ICT and Future Planning
- 등재 SCOPUS
- 발행기관 Humana Press Inc.
- 발행년도 2017
- URI http://www.dcollection.net/handler/ewha/000000156240
- 본문언어 영어
- Published As http://dx.doi.org/10.1007/978-1-4939-6857-2_2
초록/요약
Many large synthetic antibody libraries have been designed, constructed, and successfully generated high-quality antibodies suitable for various demanding applications. While synthetic antibody libraries have many advantages such as optimized framework sequences and a broader sequence landscape than natural antibodies, their sequence diversities typically are generated by random combinatorial synthetic processes which cause the incorporation of many undesired CDR sequences. Here, we describe the construction of a synthetic scFv library using oligonucleotide mixtures that contain predefined, non-combinatorially synthesized CDR sequences. Each CDR is first inserted to a master scFv framework sequence and the resulting single-CDR libraries are subjected to a round of proofread panning. The proofread CDR sequences are assembled to produce the final scFv library with six diversified CDRs. © Springer Science+Business Media LLC 2017.
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