Double intratibial injection of human tonsil-derived mesenchymal stromal cells recovers postmenopausal osteoporotic bone mass
- 주제(키워드) femoral head , intratibial injection , osteoporosis , ovariectomized , tonsil-derived mesenchymal stem cells , visceral fat
- 주제(기타) Cell & Tissue Engineering; Biotechnology & Applied Microbiology; Cell Biology; Hematology; Medicine, Research & Experimental
- 설명문(일반) [Kim, Gyungah; Jin, Yoon Mi; Yu, Yeonsil; Kim, Ha Yeong; Jo, Inho] Ewha Womans Univ, Sch Med, Dept Mol Med, Seoul 07985, South Korea; [Kim, Gyungah; Jin, Yoon Mi; Yu, Yeonsil; Kim, Ha Yeong; Jo, Inho] Ewha Womans Univ, Sch Med, Ewha Tonsil Derived Mesenchymal Stem Cells Res Ct, Seoul 07985, South Korea; [Jo, Sangmee Ahn] Dankook Univ, Dept Nanobiomed Sci, Cheonan, Chungnam, South Korea; [Jo, Sangmee Ahn] Dankook Univ, PLUS NBM Global Res Ctr Regenerat Med BK21, Cheonan, Chungnam, South Korea; [Jo, Sangmee Ahn] Dankook Univ, Dept Pharmacol, Coll Pharm, Cheonan, Chungnam, South Korea; [Park, Yoon Jeong] NIBEC, Cent Res Inst, Seoul, South Korea; [Park, Yoon Jeong] Seoul Natl Univ, Dent Res Inst, Sch Dent, Dept Dent Regenerat Biotechnol, Seoul, South Korea; [Park, Yoon Shin] Chungbuk Natl Univ, Sch Biol Sci, Coll Nat Sci, Microbiol, Cheongju 28644, Chungbuk, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 ELSEVIER SCI LTD
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000156273
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.jcyt.2018.06.008
초록/요약
Background and aims: Osteoporosis, which is a disease characterized by weakening of the bone, affects a large portion of the senior population. The current therapeutic options for osteoporosis have side effects, and there is no effective treatment for severe osteoporosis. Thus, we urgently need new treatment strategies, such as topical therapies and/or safe and effective stem cell therapies. Methods: We investigated the therapeutic potential of directly injecting human tonsil-derived mesenchymal stem cells (TMSC) into the right proximal tibias of ovariectomized postmenopausal osteoporosis model mice. Injections were given once (1x) or twice (2x) during the 3-month experimental period. At the end of the experiment, micro -computed tomographic images revealed some improvement in the proximal tibias and more significant improvement in the femoral heads of treated mice. Results: Osteogenic effect was qualitatively and quantitatively more pronounced in TMSC/2x-treated mice. Furthermore, TMSC/2x mice exhibited significant recovery of the serum osteocalcin level, which is pathologically elevated in osteoporosis, and increased serum alkaline phosphatase, which indicates bone formation. TMSC therapy was generally well tolerated and caused no apparent toxicity in the experimental mice. Moreover, TMSC therapy reduced visceral fat. Conclusion: Our results demonstrate that double injection of TMSC directly into the proximal tibia triggers recovery of osteoporosis, and thus could be a potential therapeutic approach for severe bone loss.
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