Rpd3L HDAC links H3K4me3 to transcriptional repression memory
- 주제(기타) Biochemistry & Molecular Biology
- 설명문(일반) [Lee, Bo Bae; Choi, Ahyoung; Kim, Ji Hyun; Jun, Yukyung; Woo, Hyeonju; Ha, So Dam; Yoon, Chae Young; Lee, Sanghyuk; Kim, TaeSoo] Ewha Womans Univ, Dept Life Sci, Seoul 03760, South Korea; [Lee, Bo Bae; Choi, Ahyoung; Kim, Ji Hyun; Jun, Yukyung; Woo, Hyeonju; Ha, So Dam; Yoon, Chae Young; Lee, Sanghyuk; Kim, TaeSoo] Ewha Womans Univ, Res Ctr Cellular Homeostasis, Seoul 03760, South Korea; [Hwang, Jin-Taek] Korea Food Res Inst, Wonju 55365, South Korea; [Steinmetz, Lars] European Mol Biol Lab, Genome Biol Unit, Meyerhofstr 1, D-69117 Heidelberg, Germany; [Steinmetz, Lars] Stanford Univ, Sch Med, Stanford Genome Technol Ctr, Stanford, CA 94305 USA; [Steinmetz, Lars] Stanford Univ, Sch Med, Dept Genet, Stanford, CA 94305 USA; [Buratowski, Stephen] Harvard Med Sch, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA; [Kim, Hye Young] Seoul Natl Univ, Dept Biomed Sci & Med Sci, Coll Med, Seoul 03080, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 OXFORD UNIV PRESS
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000156627
- 본문언어 영어
- Published As http://dx.doi.org/10.1093/nar/gky573
초록/요약
Transcriptional memory is critical for the faster reactivation of necessary genes upon environmental changes and requires that the genes were previously in an active state. However, whether transcriptional repression also displays 'memory' of the prior transcriptionally inactive state remains unknown. In this study, we show that transcriptional repression of similar to 540 genes in yeast occurs much more rapidly if the genes have been previously repressed during carbon source shifts. This novel transcriptional response has been termed transcriptional repression memory (TREM). Interestingly, Rpd3L histone deacetylase (HDAC), targeted to active promoters induces TREM. Mutants for Rpd3L exhibit increased acetylation at active promoters and delay TREM significantly. Surprisingly, the interaction between H3K4me3 and Rpd3L via the Pho23 PHD finger is critical to promote histone deacetylation and TREM by Rpd3L. Therefore, we propose that an active mark, H3K4me3 enriched at active promoters, instructs Rpd3L HDAC to induce histone deacetylation and TREM.
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