Skin irritation and sensitization potential of oxidative hair dye substances evaluated with in vitro, in chemico and in silico test methods
- 주제(키워드) Dermal toxicity , Hair dyes , Skin sensitization , Skin irritation , Altemative test method
- 주제(기타) Food Science & Technology; Toxicology
- 설명문(일반) [Park, Hanseul; Hwan, Jee-hyun; Lim, Kyung-Min] Ewha Womans Univ, Coll Pharm, 52 Ewhayeodae Gil, Seoul 03760, South Korea; [Han, Ji-Seok; Lee, Byoung-Seok; Kim, Yong-Bum] Korea Inst Toxicol, Dept Adv Toxicol Res, Daejeon 34114, South Korea; [Han, Ji-Seok] Chungnam Natl Univ, Coll Vet Med, Dept Vet Pathol, Daejeon, South Korea; [Joo, Kyung-Mi; Choi, Min-Seok; Cho, Sun-A] AmorePacific Corp, R&D Ctr, Yongin 17074, South Korea; [Kim, Bae-Hwan] Keimyung Univ, Food & Hlth Sci, Daegu 42601, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 PERGAMON-ELSEVIER SCIENCE LTD
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000156632
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.fct.2018.09.017
초록/요약
Permanent oxidative hair dyes are widely used but their toxicity is not well-established. Here we aimed to evaluate the skin sensitization and irritation of nine hair dye substances (MAP, MRP-N, RS, PAOX, 2,4-DAPE, 2,6-PYR, PPD, Grey HED and PM) permitted for use in EU and Korea, using in vitro and in chemico and in silico test methods. Skin sensitization was evaluated by the KeratinoSens (TM) assay, Direct Peptide Reactivity Assay (DPRA) and DEREK. Six of nine dyes tested were determined as sensitizers in common. However, the decision for MAP, RS or PAOX was diverged across assays showing 2 positives and 1 negative. Skin irritation of hair dye substances was assessed with or without 6% H2O2 on a reconstructed human epidermis, Epiderm (TM), which demonstrated that H2O2 increased the skin irritation potential of some hair dyes. PPD and PM were determined to be irritants with H2O2. Epidermal damages by hair dye and H2O2 could be further confirmed through the histology of tissue remaining after MTT assay. Collectively, our study demonstrated that hair dyes possess potential skin sensitization and irritation issues which could be further aggravated by H2O2.
more