Bacterial community analysis of stabilized soils in proximity to an exhausted mine
- 주제(키워드) Bacterial community analysis , Heavy metal , Methylobacter , Pyrosequencing , Soil stabilization
- 주제(기타) Engineering, Environmental; Environmental Sciences
- 설명문(일반) [Park, Jae Eun; Son, Ahjeong] Ewha Womans Univ, Dept Environm Sci & Engn, Seoul 03760, South Korea; [Lee, Byung-Tae] GIST, Dept Environm Sci & Engn, Gwangju 61004, South Korea; [Kim, Byung-Yong] Chun Lab Inc, Bioinformatcis Res Inst, Seoul 06725, South Korea
- 등재 SCIE, SCOPUS, KCI등재
- 발행기관 KOREAN SOC ENVIRONMENTAL ENGINEERS
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000156644
- 본문언어 영어
- Published As http://dx.doi.org/10.4491/eer.2018.040
초록/요약
Soil stabilization is a soil remediation technique that reduces the mobility of heavy metals in soils. Although it is a well-established technique, it is nonetheless essential to perform a follow-up chemical assessment via a leaching test to evaluate the immobilization of heavy metals in the soil matrix. Unfortunately, a standard chemical assessment is not sufficient for evaluation of the biological functional state of stabilized soils slated for agricultural use. Therefore, it is useful to employ a pyrosequencing-based microbial community analysis for the purpose. In this study, a recently stabilized site in the proximity of an exhausted mine was analyzed for bacterial diversity, richness, and relative abundance as well as the effect of environmental factors. Based on the Shannon and Chao1 indices and rarefaction curves, the results showed that the stabilized layer exhibited lower bacterial diversity than control soils. The prevalence of dominant bacterial populations was examined in a hierarchical manner. Relatively high abundances of Proteobacteria and Methylobacter tundripaluilum were observed in the stabilized soil. In particular, there was substantial abundance of the Methylobacter genus, which is known for its association with heavy metal contamination. The study demonstrated the efficacy of (micro)biological assessment for aiding in the understanding and post-management of stabilized soils.
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