Quantitative screening for endocrine-disrupting bisphenol A in consumer and household products using NanoAptamer assay
- 주제(키워드) Bisphenol A , Endocrine disrupting chemicals , Quantum dots , Aptamer , Aptasensor
- 주제(기타) Environmental Sciences
- 설명문(일반) [Lim, Hyun Jeong; Lee, Eun-Hee; Lee, Sang-Don; Son, Ahjeong] Ewha Womans Univ, Dept Environm Sci & Engn, Seoul 03760, South Korea; [Yoon, Yeomin] Univ South Carolina, Dept Civil & Environm Engn, Columbia, SC 29208 USA
- 등재 SCIE, SCOPUS
- 발행기관 PERGAMON-ELSEVIER SCIENCE LTD
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000156649
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.chemosphere.2018.07.125
초록/요약
The NanoAptamer assay is a bisphenol A (BPA) quantification method that uses magnetic beads, quantum dot nanoparticles, and a BPA-specific aptamer. In this study, screening of various consumer and household products for BPA was demonstrated utilizing the NanoAptamer assay. First, the experimental conditions suitable for BPA detection using the NanoAptamer assay were examined in terms of incubation time, temperature, and buffer composition. The range of BPA quantification via the NanoAptamer assay was determined to be 0.005-1000 ng/mL of BPA. The selectivity was confirmed by detecting BPA in an analog mixture containing bisphenol S and bisphenol F. Finally, a leaching experiment using 20 consumer and household products classified into 4 categories was performed to demonstrate the capability of the NanoAptamer assay for BPA detection. The experiment was validated by high-performance liquid chromatography analysis (correlation coefficient, r = 0.99). (C) 2018 Elsevier Ltd. All rights reserved.
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