The Implications of Fragmented Genomic DNA Size Range on the Hybridization Efficiency in NanoGene Assay
- 주제(키워드) DNA fragmentation , optimum size , quantification capability , hybridization efficiency , magnetic beads , quantum dots
- 주제(기타) Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation
- 설명문(일반) [Wang, Xiaofang] Auburn Univ, Dept Civil Engn, Auburn, AL 36849 USA; [Chua, Beelee] Korea Univ, Sch Elect Engn, Seoul 02841, South Korea; [Son, Ahjeong] Ewha Womans Univ, Dept Environm Sci & Engn, Seoul 03760, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 MDPI
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000156650
- 본문언어 영어
- Published As http://dx.doi.org/10.3390/s18082646
초록/요약
DNA hybridization-based assays are well known for their ability to detect and quantify specific bacteria. Assays that employ DNA hybridization include a NanoGene assay, fluorescence in situ hybridization, and microarrays. Involved in DNA hybridization, fragmentation of genomic DNA (gDNA) is necessary to increase the accessibility of the probe DNA to the target gDNA. However, there has been no thorough and systematic characterization of different fragmented gDNA sizes and their effects on hybridization efficiency. An optimum fragmented size range of gDNA for the NanoGene assay is hypothesized in this study. Bacterial gDNA is fragmented via sonication into different size ranges prior to the NanoGene assay. The optimum size range of gDNA is determined via the comparison of respective hybridization efficiencies (in the form of quantification capabilities). Different incubation durations are also investigated. Finally, the quantification capability of the fragmented (at optimum size range) and unfragmented gDNA is compared.
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