A Two-Photon Fluorescent Probe for Imaging Endogenous ONOO(- )near NMDA Receptors in Neuronal Cells and Hippocampal Tissues
- 주제(기타) Chemistry, Analytical
- 설명문(일반) [Lee, Dayoung; Ko, Gyeongju; Kim, Dayoung; Nam, Sang-Jip; Yoon, Juyoung] Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea; [Lim, Chang Su; Cho, Myoung Ki; Kim, Hwan Myung] Ajou Univ, Dept Energy Syst Res, Suwon 443749, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 AMER CHEMICAL SOC
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000156657
- 본문언어 영어
- Published As http://dx.doi.org/10.1021/acs.analchem.8b01960
초록/요약
In this study, we developed a two-photon fluorescent probe for detection of peroxynitrite (ONOO-) near the N-methyl-D-aspartate (NMDA) receptor. This naphthalimide-based probe contains a boronic acid reactive group and an ifenprodil-like tail, which serves as an NMDA receptor targeting unit. The probe displays high sensitivity and selectivity, along with a fast response time in aqueous solution. More importantly, the probe can be employed along with two-photon fluorescence microscopy to detect endogenous ONOO- near NMDA receptors in neuronal cells as well as in hippocampal tissues. The results suggest that the probe has the potential of serving as a useful imaging tool for studying ONOO- related diseases in the nervous system.
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