Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum strains from empty fruit bunch biosugar solution
- 주제(키워드) Corynebacterium glutamicum , Co-utilization , Xylose , Gamma-aminobutyrate , Empty fruit bunch
- 주제(기타) Biotechnology & Applied Microbiology
- 설명문(일반) [Baritugo, Kei-Anne; David, Yokimiko; Park, Si Jae] Ewha Womans Univ, Div Chem Engn & Mat Sci, 52 Ewhayeodae Gil, Seoul 03760, South Korea; [Kim, Hee Taek; Khang, Tae Uk; Hyun, Sung Min; Kang, Kyoung Hee; Yu, Ju Hyun; Joo, Jeong Chan] Korea Res Inst Chem Technol, Biobased Chem Res Ctr, Adv Convergent Chem Div, POB 107,141 Gajeong Ro, Daejeon 34602, South Korea; [Choi, Jong Hyun; Song, Jae Jun] Korea Res Inst Biosci & Biotechnol, Microbial Biotechnol Res Ctr, Jeonbuk Branch Inst, 181 Ipsin Gil, Jeongeup 56212, Jeonbuk, South Korea
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- 발행기관 BMC
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000156903
- 본문언어 영어
- Published As http://dx.doi.org/10.1186/s12934-018-0977-9
초록/요약
Background: Recent interest has been focused on the production of platform chemicals from renewable biomass due to increasing concerns on global warming and depletion of fossil fuel reserves. Microbial production of platform chemicals in biorefineries has been suggested to be a promising solution for these problems. Gamma-aminobutyrate (GABA), a versatile bulk chemical used in food and pharmaceutical industry, is also used as a key monomer for nylon 4. GABA can be biologically produced by decarboxylation of glutamate. Results: In this study, we examined high glutamate-producing Corynebacterium glutamicum strains as hosts for enhanced production of GABA from glucose and xylose as carbon sources. An Escherichia coli gadB mutant with a broad pH range of activity and E. coli xylAB genes were expressed under the control of a synthetic H36 promoter. When empty fruit bunch (EFB) solution was used as carbon source (45 g/L glucose and 5 g/L xylose), 12.54 +/- 0.07 g/L GABA was produced by recombinant C. glutamicum H36GD1852 expressing E. coli gadB mutant gene and xylAB genes. Batch fermentation of the same strain resulted in the production of 35.47 g/L of GABA when EFB solution was added to support 90 g/L glucose and 10 g/L xylose. Conclusions: This is the first report of GABA production by recombinant C. glutamicum strains from co-utilization of glucose and xylose from EFB solution. Recombinant C. glutamicum strains developed in this study should be useful for an efficient and sustainable production of GABA from lignocellulosic biomasses.
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