Characterization of a Whole-Cell Biotransformation Using a Constitutive Lysine Decarboxylase from Escherichia coli for the High-Level Production of Cadaverine from Industrial Grade l-Lysine
- 주제(키워드) Cadaverine , l-Lysine , Lysine decarboxylase , Reusability , Whole-cell biotransformation
- 관리정보기술 faculty
- 등재 SCIE, SCOPUS
- 발행기관 Humana Press Inc.
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000156904
- 본문언어 영어
- Published As http://dx.doi.org/10.1007/s12010-018-2696-4
초록/요약
Cadaverine is used for the synthesis of the novel bio-polyamides 54, 56, and 510. Here, we examine the feasibility of using a lysine decarboxylase (LdcC) from Escherichia coli for high-level production of cadaverine. After sequential optimization of whole-cell biotransformation conditions, recombinant E. coli-overexpressing LdcC (EcLdcC) could produce 1.0 M cadaverine from 1.2 M crude l-lysine solution after 9 h. EcLdcC retained a higher cadaverine yield after being reused 10 times at acidic and alkaline pH values than that of a recombinant E. coli strain overexpressing an inducible lysine decarboxylase (CadA), a conventional cadaverine producer (90 vs. 51% at pH 6 and 55 vs. 15% at pH 8). This study reveals that EcLdcC is a promising whole-cell biocatalyst for the bio-based production of cadaverine from industrial grade l-lysine in comparison to EcCadA. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
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