Pretreatment of kenaf (Hibiscus cannabinus L.) biomass feedstock for polyhydroxybutyrate (PHB) production and characterization
- 주제(키워드) Kenaf biomass , Green liquor pretreatment , Poly(3-hydroxybutyrate) , Ralstonia eutropha ATCC 17699 , Enzymatic hydrolysis
- 주제(기타) Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels
- 설명문(일반) [Saratale, Rijuta Ganesh; Kadam, Avinash] Dongguk Univ Seoul, Res Inst Biotechnol & Med Converged Sci, Goyang Si 10326, Gyeonggido, South Korea; [Saratale, Ganesh Dattatraya; Shin, Han Seung] Dongguk Univ Seoul, Dept Food Sci & Biotechnol, Goyang Si 10326, Gyeonggido, South Korea; [Cho, Si Kyung; Ghodake, Gajanan S.] Dongguk Univ Seoul, Dept Biol & Environm Sci, Goyang Si 10326, Gyeonggido, South Korea; [Kim, Dong Su] Ewha Womans Univ, Dept Environm Sci & Engn, Seoul 120750, South Korea; [Kumar, Gopalakrishanan] Univ Stavanger, Fac Sci & Technol, Inst Chem Biosci & Environm Engn, Box 8600 Forus, N-4036 Stavanger, Norway; [Bharagava, Ram Naresh] Babasaheb Bhimrao Ambedkar Univ, Sch Environm Sci, Dept Microbiol, Vidya Vihar 226025, Uttar Pradesh, India; [Banu, Rajesh] Anna Univ, Dept Civil Engn, Reg Campus, Tirunelveli 627007, Tamil Nadu, India
- 등재 SCIE, SCOPUS
- 발행기관 ELSEVIER SCI LTD
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000159693
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.biortech.2019.02.083
- PubMed https://pubmed.ncbi.nlm.nih.gov/30851577
초록/요약
Kenaf biomass (KB) was employed as feedstock for the synthesis of polyhydroxybutyrate (PHB) using Ralstonia eutropha to replace conventional petroleum-derived polymers. Various pretreatments followed by enzymatic saccharification were applied to release monomeric sugars from KB for PHB production. The effects of increasing concentration of Na2CO3 + Na2SO3 (NaC+ NaS) pretreated KB hydrolysates (20-40 g/L) on PHB production were investigated. NaC+ NaS pretreated KB hydrolysates (30 g/L) exhibited maximum 70.0% PHA accumulation, with PHB titers of 10.10 g/L and PHB yields of about 0.488 g/g of reducing sugar produced within 36 h of fermentation. PHA accumulation, PHB yield and R. eutropha growth performance using KB hydrolysates were found to be comparable with those of synthetic sugar mixture. Characterization of the produced PHB in terms of crystalline structure, and thermal properties was done using various analytical techniques and results coincide with standard PHB. Thus, green liquor pretreated KB hydrolysates deliver a promising and economically feasible carbon substrate for PHB production.
more