Intra-articular delivery of synovium-resident mesenchymal stem cells via BMP-7-loaded fibrous PLGA scaffolds for cartilage repair
- 주제(키워드) Synovium-resident mesenchymal stem cells , Bone morphogenetic protein-7 , Combined delivery , PLGA fibrous scaffold , Articular cartilage repair
- 주제(기타) Chemistry, Multidisciplinary; Pharmacology & Pharmacy
- 설명문(일반) [Kim, Hee Jung; Han, Min Ah.; Shin, Ji Young; Jeon, Jeong Hwa; Lee, Seung Jin] Ewha Womans Univ, Coll Pharm, Dept Pharm, 52 Ewhayeodae Gil, Seoul 03760, South Korea; [Yoon, Mi Yun] Dongnam Hlth Univ, Dept Cosmetol, 50 Cheoncheon Ro 74 Gil, Suwon 16328, Gyeonggi Do, South Korea; [Kim, Han-Jun; Choi, Eun-Ji; Do, Sun Hee] Konkuk Univ, Coll Vet Med, Dept Vet Clin Pathol, 120 Neungdong Ro, Seoul 05029, South Korea; [Yang, Victor C.; He, Huining] Tianjin Med Univ, Sch Pharm, Tianjin Key Lab Technol Enabling Dev Clin Therape, Tianjin 300070, Peoples R China; [Yang, Young-Il] Inje Univ, Coll Med, Paik Inst Clin Res, 75 Bokji Ro, Busan 47392, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 ELSEVIER SCIENCE BV
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000159729
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.jconrel.2019.04.002
- PubMed https://pubmed.ncbi.nlm.nih.gov/30954618
초록/요약
Delivery of synovium-resident mesenchymal stem cells (synMSCs) to cartilage defect site might provide a novel therapeutic modally for treatment of articular cartilage diseases. However, low isolation efficiency of synMSCs limits their therapeutic application. Niche-preserving non-enzymatic isolation of synMSCs was firstly attempted by employing micro-organ culture system based on recapitulating tissue-specific homeostasis ex vivo. The isolated synMSCs retained superior long-term growth competency, proliferation and chondrogenic potential to bone marrow-derived MSCs (BMSCs). It was noted that synMSCs demonstrated 9-fold increase in cartilaginous micro-tissue formation and 13-fold increase in sulfated proteoglycans deposition compared to BMSCs. For delivery of synMSCs, fibrous PLGA scaffolds were specifically designed for full-thickness osteochondral defects in rabbits. The scaffolds provided effective micro-environment for growth and host-integration of synMSCs. Combined delivery of synMSCs with bone morphogenetic proteins-7 (BMP-7) was designed to achieve synergistic therapeutic efficacy. BMP-7-loaded PLGA nanoparticles electrosprayed onto the scaffolds released BMP-7 over 2 weeks to conform with its aimed role in stimulating early stage endochondral ossification. Scaffold-supported combined administration of synMSCs with BMP-7 resulted in high proteoglycan and collagen type II induction and thick hyaline cartilage formation. Intra-articular co-delivery of synMSCs with BMP-7 via fibrous PLGA scaffolds may be a promising therapeutic modality for articular cartilage repair.
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