Dimerized, Not Monomeric, Translationally Controlled Tumor Protein Induces Basophil Activation and Mast Cell Degranulation in Chronic Urticaria
- 주제(키워드) Chronic urticaria , Translationally-controlled tumor protein , Basophils , Mast cells
- 주제(기타) Immunology
- 설명문(일반) [Ulambayar, Bastsetseg; Yang, Eun-Mi; Park, Hae-Sim; Ye, Young-Min] Ajou Univ, Dept Allergy & Clin Immunol, Sch Med, 164 World Cup Ro, Suwon 16499, South Korea; [Lee, Heewon; Lee, Kyunglim] Ewha Womans Univ, Coll Pharm, Grad Sch Pharmaceut Sci, Seoul 03760, South Korea
- 등재 SCIE, SCOPUS, KCI등재
- OA유형 Green Published, hybrid, Green Submitted
- 발행기관 KOREA ASSOC IMMUNOLOGISTS
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000159737
- 본문언어 영어
- Published As http://dx.doi.org/10.4110/in.2019.19.e20
- PubMed https://pubmed.ncbi.nlm.nih.gov/31281717
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Translationally controlled tumor protein (TCTP) is also known as histamine releasing factor as it has the ability to activate mast cells. To investigate the role of TCTP in the pathogenesis of chronic spontaneous urticaria (CSU), we evaluated serum level of TCTP and effect of TCTP on basophil and mast cell degranulation. TCTP levels in the sera from 116 CSU patients and 70 normal healthy controls (NCs) were measured by ELISA. CD203c expression on basophils from CSU patients and 13-hexosaminidase release from Laboratory of Allergic Disease 2 mast cells were measured upon stimulation monomeric and dimeric TCTP. Non-reducing Western blot analysis was used for detecting dimeric TCTP. No difference was observed in serum TCTP levels between CSU patients and NCs (p=0.676). However, dimeric TCTP intensity on Western blot was stronger in CSU patients than in NCs. TCTP levels were higher in patients with severe CSU (p=0.049) and with IgG positivity to Fc epsilon RI alpha (p=0.038). A significant positive correlation was observed between TCTP and eosinophil cationic protein levels (Spearman's rho=0.341; p=0.001). Both basophil and mast cell degranulation were significantly increased after stimulation with dimeric TCTP, but not with monomic TCTP. The ability of TCTP to activate basophil and mast cells is dependent on dimerization, suggesting that the inhibition of TCTP dimerization can be a therapeutic option for CSU. Association between TCTP levels and the presence of IgG to high affinity Fc epsilon receptor I alpha subunit in CSU patients indicates that autoimmune mechanisms may be involved in the dimerization of TCTP.
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