Tonsil-derived mesenchymal stem cell-embedded in situ crosslinkable gelatin hydrogel therapy recovers postmenopausal osteoporosis through bone regeneration
- 주제(기타) Multidisciplinary Sciences
- 설명문(일반) [Kim, Gyungah; Jin, Yoon Mi; Jo, Inho] Ewha Womans Univ, Coll Med, Dept Mol Med, Seoul, South Korea; [Kim, Gyungah; Jin, Yoon Mi; Ryu, Kyung-Ha; Jo, Inho] Ewha Womans Univ, Coll Med, Ewha Tonsil Derived Mesenchymal Stem Cells Res Ct, Seoul, South Korea; [Park, Yoon Shin; Choi, Da Hyeon] Chungbuk Natl Univ, Coll Nat Sci, Sch Biol Sci, Microbiol, Cheongju, South Korea; [Lee, Yunki; Park, Ki Dong] Ajou Univ, Dept Mol Sci & Technol, Suwon, South Korea; [Ryu, Kyung-Ha] Ewha Womans Univ, Coll Med, Dept Pediat, Seoul, South Korea; [Park, Yoon Jeong] Seoul Natl Univ, Sch Dent, Dent Res Inst, Dept Dent Regenerat Biotechnol, Seoul, South Korea; [Park, Yoon Jeong] NIBEC, Cent Res Inst, Seoul, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 PUBLIC LIBRARY SCIENCE
- 발행년도 2018
- URI http://www.dcollection.net/handler/ewha/000000159784
- 본문언어 영어
- Published As http://dx.doi.org/10.1371/journal.pone.0200111
초록/요약
We investigated therapeutic potential of human tonsil-derived mesenchymal stem cells (TMSC) subcutaneously delivered to ovariectomized (OVX) mice for developing more safe and effective therapy for osteoporosis. TMSC were isolated from tonsil tissues of children undergoing tonsillectomy, and TMSC-embedded in situ crosslinkable gelatin-hydroxyphenyl propionic acid hydrogel (TMSC-GHH) or TMSC alone were delivered subcutaneously to the dorsa of OVX mice. After 3 months, three-dimensionally reconstructed micro-computed tomographic images revealed better recovery of the femoral heads in OVX mice treated with TMSC-GHH. Serum osteocalcin and alkaline phosphatase were also recovered, indicating bone formation only in TMSC-GHH-treated mice, and absence in hypercalcemia or other severe macroscopic deformities showed biocompatibility of TMSC-GHH. Additionally, visceral fat reduction effects by TMSC-GHH further supported their therapeutic potential. TMSC provided therapeutic benefits toward osteoporosis only when embedded in GHH, and showed potential as a supplement or alternative to current therapies.
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