Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells
- 주제(키워드) reaction-based fluorescent probe , alkaline phosphatase , cell imaging , fluorescence , colorimetric
- 주제(기타) Chemistry, Multidisciplinary
- 설명문(일반) [Gwynne, Lauren; Gardiner, Jordan E.; Williams, George T.; Lowe, John P.; Jenkins, A. Toby A.; Bull, Steven D.; James, Tony D.] Univ Bath, Dept Chem, Bath, Avon, England; [Sedgwick, Adam C.; Sessler, Jonathan L.] Univ Texas Austin, Dept Chem, Austin, TX 78712 USA; [Kim, Gyoungmi; Yoon, Juyoung] Ewha Womans Univ, Dept Chem & Nano Sci, Seoul, South Korea; [Maillard, Jean-Yves] Cardiff Univ, Cardiff Sch Pharm & Pharmaceut Sci, Cardiff, S Glam, Wales
- 등재 SCIE, SCOPUS
- OA유형 Green Published, gold
- 발행기관 FRONTIERS MEDIA SA
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000159910
- 본문언어 영어
- Published As http://dx.doi.org/10.3389/fchem.2019.00255
- PubMed https://pubmed.ncbi.nlm.nih.gov/31119120
초록/요약
A long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence "turn on" (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.
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