Oligo(ethylene glycol)-Functionalized Ratiometric Fluorescent Probe for the Detection of Hydrazine in Vitro and in Vivo
- 주제(기타) Chemistry, Analytical
- 설명문(일반) [Li, Jun; Cui, Yuanchao; Bi, Chenxi; Feng, Shaoqiong; Yu, Fengzhen; Yuan, En; Xu, Shengzhen; Wei, Dengguo] Huazhong Agr Univ, Dept Chem, Coll Sci, Wuhan 430070, Hubei, Peoples R China; [Hu, Zhe; Wei, Dengguo] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China; [Yoon, Juyoung] Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea; [Sun, Qi] Wuhan Inst Technol, Minist Educ, Key Lab Green Chem Proc, Wuhan 430205, Hubei, Peoples R China; [Sun, Qi] Wuhan Inst Technol, Sch Chem & Environm Engn, Wuhan 430205, Hubei, Peoples R China
- 등재 SCIE, SCOPUS
- 발행기관 AMER CHEMICAL SOC
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000159914
- 본문언어 영어
- Published As http://dx.doi.org/10.1021/acs.analchem.9b01223
- PubMed https://pubmed.ncbi.nlm.nih.gov/31062588
초록/요약
Hydrazine induced toxicity causes serious harm to the health of humans. The detection of N2H4 in vitro and in vivo has attracted a great deal of attention, especially in the context of fluorescent probes. Although some fluorescent N2H4 probes have been reported, only a few operate in purely aqueous media and, as a result, require the use of organic cosolvents which hinders their use in analysis of real samples. In addition, most of the current N2H4 probes are either "off on" or "on off' types, in which it is difficult to eliminate interference from background fluorescence commonly occurring in in vitro and in vivo systems. Furthermore, some probes are unable to differentiate hydrazine from other organic amines. To address the above problems, we developed a novel oligo(ethylene glycol)-functionalized fluorescent probe for the detection of N2H4. The probe, which has a donor-pi-acceptor (D-pi-A)-type structure, is water-soluble, and it can be utilized to selectively detect N2H4 in both colorimetric and ratiometric mode. Furthermore, the probe is able to differentiate hydrazine from other organic amines and can be used to detect hydrazine vapor and for imaging A549 cells and zebrafish.
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