Molecular characteristics and induction profiles of hypoxia-inducible factor-1 alpha and other basic helix-loop-helix and Per-Arnt-Sim domain-containing proteins identified in a carcinogenic liver fluke Clonorchis sinensis
- 주제(키워드) bHLH-PAS proteins , Clonorchis sinensis , HIF-1 alpha , hypoxia , oxygen homeostasis
- 주제(기타) Parasitology
- 설명문(일반) [Kim, Seon-Hee; Oh, Gyu-Seok; Bae, Young-An] Gachon Univ, Dept Microbiol, Coll Med, 191 Hambakmoe Ro, Incheon, South Korea; [Sohn, Woon-Mok] Gyeongsang Natl Univ, Coll Med, Dept Parasitol, Inst Hlth Sci, Jinju 52727, South Korea; [Lee, Kihyun] Chung Ang Univ, Ctr Antibiot Resistome, Dept Syst Biotechnol, Anseong 17546, South Korea; [Yang, Hyun-Jong] Ewha Womans Univ, Sch Med, Dept Parasitol, Seoul 03760, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 CAMBRIDGE UNIV PRESS
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000159917
- 본문언어 영어
- Published As http://dx.doi.org/10.1017/S0031182018001245
- PubMed https://pubmed.ncbi.nlm.nih.gov/30070186
초록/요약
Clonorchis sinensis (C. sinensis), a trematode parasite that invades the hypoxic hepatobiliary tract of vertebrate hosts requires a considerable amount of oxygen for its sexual reproduction and energy metabolism. However, little is known regarding the molecular mechanism of C. sinensis involved in the adaptation to the hypoxic environments. In this study, we investigated the molecular structures and induction patterns of hypoxia-inducible factor-1 alpha (HIF-1 alpha) and other basic helix-loop-helix and Per-Arnt-Sim (bHLH-PAS) domain-containing proteins such as HIF-1 beta, single-minded protein and aryl hydrocarbon receptor, which might prompt adaptive response to hypoxia, in C. sinensis. These proteins possessed various bHLH-PAS family-specific domains. Expression of C. sinensis HIF-1 alpha (CsHIF-1 alpha) was highly induced in worms which were either exposed to a hypoxic condition or co-incubated with human cholangiocytes. In addition to oxygen, nitric oxide and nitrite affected the CsHIF-1 alpha expression depending on the surrounding oxygen concentration. Treatment using a prolyl hydroxylase-domain protein inhibitor under 20%-oxygen condition resulted in an increase in the CsHIF-1 alpha level. Conversely, the other bHLH-PAS genes were less responsive to these exogenous stimuli. We suggest that nitrite and nitric oxide, as well as oxygen, coordinately involve in the regulation of HIF-1 alpha expression to adapt to the hypoxic host environments in C. sinensis.
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