Alveolar Macrophages Treated With Bacillus subtilis Spore Protect Mice Infected With Respiratory Syncytial Virus A2
- 주제(키워드) respiratory syncytial virus , Bacillus subtilis , spore , alveolar macrophage activation , MyD88
- 주제(기타) Microbiology
- 설명문(일반) [Hong, Ji Eun; Kye, Yoon-Chul; Park, Sung-Moo; Cheon, In Su; Yun, Cheol-Heui] Seoul Natl Univ, Res Inst Agr & Life Sci, Dept Agr Biotechnol, Seoul, South Korea; [Park, Sung-Moo; Yun, Cheol-Heui] Seoul Natl Univ, Ctr Food & Bioconvergence, Seoul, South Korea; [Cheon, In Su; Song, Man Ki] Int Vaccine Inst, Dept Mol Vaccinol, Div Sci Lab, Seoul, South Korea; [Chu, Hyuk] Korea Ctr Dis Control & Prevent, Natl Inst Hlth, Div Zoonoses, Ctr Immunol & Pathol, Cheongju, South Korea; [Park, Byung-Chul; Yun, Cheol-Heui] Seoul Natl Univ, Inst Green Bio Sci & Technol, Seoul, South Korea; [Park, Yeong-Min] Konkuk Univ, Sch Med, Dept Immunol, Chungju, South Korea; [Chang, Jun] Ewha Womans Univ, Grad Sch Pharmaceut Sci, Seoul, South Korea; [Cho, Jae-Ho] Acad Immunol & Microbiol, Inst Basic Sci, Pohang, South Korea; [Han, Seung Hyun] Seoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, DRI, Seoul, South Korea; [Han, Seung Hyun] Seoul Natl Univ, Sch Dent, BK21 Plus Program, Seoul, South Korea
- 등재 SCIE, SCOPUS
- OA유형 gold, Green Published
- 발행기관 FRONTIERS MEDIA SA
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000160045
- 본문언어 영어
- Published As http://dx.doi.org/10.3389/fmicb.2019.00447
- PubMed https://pubmed.ncbi.nlm.nih.gov/30930867
초록/요약
Respiratory syncytial virus (RSV) is a major pathogen that infects lower respiratory tract and causes a common respiratory disease. Despite serious pathological consequences with this virus, effective treatments for controlling RSV infection remain unsolved, along with poor innate immune responses induced at the initial stage of RSV infection. Such a poor innate defense mechanism against RSV leads us to study the role of alveolar macrophage (AM) that is one of the primary innate immune cell types in the respiratory tract and may contribute to protective responses against RSV infection. As an effective strategy for enhancing anti-viral function of AM, this study suggests the intranasal administration of Bacillus subtilis spore which induces expansion of AM in the lung with activation and enhanced production of inflammatory cytokines along with several genes associated with M1 macrophage differentiation. Such effect by spore on AM was largely dependent on TLR-MyD88 signaling and, most importantly, resulted in a profound reduction of viral titers and pathological lung injury upon RSV infection. Taken together, our results suggest a protective role of AM in RSV infection and its functional modulation by B. subtilis spore, which may be a useful and potential therapeutic approach against RSV.
more