Characterization of polymorphic loci for two cicada species: Cryptotympana atrata and Hyalessa fuscata (Hemiptera: Cicadoidae)
- 주제(키워드) Polymorphic loci , SSR , Next-generation sequencing , Cryptotympana atrata , Hyalessa fuscata
- 주제(기타) Biochemistry & Molecular Biology
- 설명문(일반) [Hoa Quynh Nguyen; Chae, Soyeon; Jang, Yikweon] Ewha Womans Univ, Interdisciplinary Program EcoCreat, Seoul, South Korea; [Kim, Erick] Univ Calif Los Angeles, Dept Geog, Los Angeles, CA 90024 USA; [Jang, Yikweon] Ewha Womans Univ, Dept Life Sci, Ewhayeodae Gil 52, Seoul 03760, South Korea; [Jang, Yikweon] Ewha Womans Univ, Div EcoSci, Ewhayeodae Gil 52, Seoul 03760, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 SPRINGER
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000160095
- 본문언어 영어
- Published As http://dx.doi.org/10.1007/s11033-018-4571-3
- PubMed https://pubmed.ncbi.nlm.nih.gov/30603951
초록/요약
The cicada species Cryptotympana atrata and Hyalessa fuscata are abundant in metropolitan Seoul where their population densities are positively correlated with local urban heat island intensities. Here, we characterized two panels of polymorphic markers for both species. A set of 21 microsatellite markers previously developed for a Chinese population of C. atrata were tested for the Korean population. Fifteen of the tested loci were found to be polymorphic in the target population. The number of alleles per locus ranged from 2 to 12, with observed and expected heterozygosities ranging from 0.2 to 1 and 0.06667 to 0.92644, respectively. Four of the tested loci were moderately polymorphic, and the rest were highly polymorphic. For H. fuscata, a novel panel of microsatellite markers was generated using a next-generation sequencing technique and 18 polymorphic loci were identified in the target population. The number of alleles per locus was between 3 and 16, with observed and expected heterozygosities ranging from 0.02222 to 0.97778 and 0.16804 to 0.86181, respectively. Polymorphism level was low in one locus, moderate in five loci, and high in the remaining loci. The microsatellite markers described in this study will be useful for identifying genetic structure and genetic differentiation among populations of C. atrata and H. fuscata in metropolitan Seoul.
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