L-histidine and L-carnosine accelerate wound healing via regulation of corticosterone and PI3K/Akt phosphorylation in D-galactose-induced aging models in vitro and in vivo
- 주제(키워드) Corticosterone , D-galactose , L-carnosine , L-histidine , Skin aging , Wound healing
- 등재 SCIE, SCOPUS
- 발행기관 Elsevier Ltd
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000160183
- 본문언어 영어
- Published As http://dx.doi.org/10.1016/j.jff.2019.04.060
초록/요약
Impaired skin wound healing in the elderly can lead to medical issues and increased mortality. Although L-histidine and L-carnosine are potent anti-aging amino acids, the wound healing effects of these amino acids in aging remain to be elucidated. Here, we investigated the regenerative potential of L-histidine and L-carnosine in in vitro and in vivo aging models. L-histidine (1 mM), L-carnosine (10 mM), or a combination improved proliferation, migration, senescence, and epithelial-mesenchymal transition (EMT)in D-galactose-induced aged keratinocytes. An in vivo mouse aging model was established with injection of D-galactose (s.c. 500 mg/kg)daily for eight weeks. Supplementation with L-histidine (2 g/L), L-carnosine (2 g/L), or a combination improved collagen and wound healing with EMT markers, E-cadherin, N-cadherin, and MMP-2. These effects were concomitant with reduced circulating levels of corticosterone and increased PI3K/Akt phosphorylation. These results suggest that L-histidine and L-carnosine have the potential to facilitate wound healing in aging skin. © 2019 Elsevier Ltd
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