Walnut phenolic extract inhibits nuclear factor kappaB signaling in intestinal epithelial cells, and ameliorates experimental colitis and colitis-associated colon cancer in mice
- 주제(키워드) Walnut , Inflammatory bowel disease , Nuclear factor kappaB , Colon cancer
- 주제(기타) Nutrition & Dietetics
- 설명문(일반) [Koh, Seong-Joon; Choi, Youn-I; Kim, Ji Won; Kim, Byeong Gwan; Lee, Kook Lae] Seoul Natl Univ, Boramae Hosp, Dept Internal Med, Coll Med, 5 Gil 20,Boramae Rd, Seoul 156707, South Korea; [Kim, Yuri; Kim, Yoo-Sun] Ewha Womans Univ, Dept Nutr Sci & Food Management, Seoul, South Korea; [Choi, Sang Woon] CHA Univ, Sch Med, Chaum Life Ctr, Seoul, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 SPRINGER HEIDELBERG
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000160184
- 본문언어 영어
- Published As http://dx.doi.org/10.1007/s00394-018-1704-3
- PubMed https://pubmed.ncbi.nlm.nih.gov/29744610
초록/요약
PurposeWalnuts (Juglans regia) are known to have anti-cancer and immunomodulatory effects. However, little information is available on the effects of walnut phenolic extract (WPE) on intestinal inflammation and colitis-associated colon cancer.MethodsCOLO205 cells were pretreated with WPE and then stimulated with tumor necrosis factor (TNF)-. In the acute colitis model, wild type mice (C57BL/6) were administered 4% dextran sulfate sodium (DSS) for 5 days. In the chronic colitis model, interleukin (IL)-10(-/-) mice were administered with either the vehicle or WPE (20mg/kg) by oral gavage daily for 2 weeks. In an inflammation-associated tumor model, wild type mice were administered a single intraperitoneal injection of azoxymethane followed by three cycles of 2% DSS for 5 days and 2 weeks of free water consumption.ResultsWPE significantly inhibited IL-8 and IL-1 expression in COLO205 cells. WPE attenuated both the TNF--induced IB phosphorylation/degradation and NF-B DNA binding activity. The administration of oral WPE significantly reduced the severity of colitis in both acute and chronic colitis models, including the IL-10(-/-) mice. In immunohistochemical staining, WPE attenuated NF-B signaling in the colons of both colitis models. Finally, WPE also significantly reduced tumor development in a murine model of colitis-associated colon cancer (CAC).ConclusionsWPE ameliorates acute and chronic colitis and CAC in mice, suggesting that WPE may have potentials for the treatment of inflammatory bowel disease.
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