Carbon-Fiber Based Microelectrode Array Embedded with a Biodegradable Silk Support for In Vivo Neural Recording
- 주제(키워드) Carbon Fiber , Silk Fibroin , Neural Recording , Tissue Response
- 주제(기타) Medicine, General & Internal
- 설명문(일반) [Lee, Yena; Jun, Sang Beom] Ewha Womans Univ, ELTEC Coll Engn, Dept Elect & Elect Engn, 52 Ewhayeodae Gil, Seoul 03760, South Korea; [Kong, Chanho; Chang, Jin Woo] Yonsei Univ, Dept Neurosurg, Coll Med, Seoul, South Korea; [Jun, Sang Beom] Ewha Womans Univ, Coll Scranton, Dept Brain & Cognit Sci, Seoul, South Korea
- OA유형 Green Published, Green Submitted, gold
- 발행기관 KOREAN ACAD MEDICAL SCIENCES
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000160223
- 본문언어 영어
- Published As http://dx.doi.org/10.3346/jkms.2019.34.e24
- PubMed https://pubmed.ncbi.nlm.nih.gov/30686948
- 저작권 이화여자대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Background: Recently, carbon fibers have been utilized to develop a depth-type microelectrode array for chronic neural recording. Since the diameter of carbon fibers is smaller than the conventional electrodes made of metal wires or microfabricated silicon, the carbon fiber electrodes showed an improved capability for chronic neural recording with less tissue damages. However, the carbon fiber based microelectrodes have a limitation of short insertion depth due to a low stiffness. Methods: We proposed a carbon fiber based microelectrode array embedded with a mechanical support structure to facilitate the penetration into the deeper brain. The support is made of biodegradable silk fibroin to reduce the reactive tissue responses. The 4-channel carbon fiber based microelectrode arrays were fabricated and accessed in terms of electrochemical impedance, recording capability for 1-month implantation in rat hippocampi. The electrodes with tungsten supports were fabricated and tested as a control group. Immunohistochemical analysis was performed to identify the reactive glial responses. Results: The carbon fiber based electrode arrays with silk supports showed about 2-fold impedance increase 2 weeks after implantation while the number of active electrodes decreased simultaneously. However, after 1 month, the electrode impedance decreased back to its initial value and the percentage of active electrodes also increased above 70%. Immunohistochemical staining clearly showed that the electrodes with silk supports induced less reactive glial responses than that with tungsten supports. Conclusion: The proposed carbon fiber based microelectrode array is expected to be used for long-term in vivo neural recording from deep brain regions with the minimized reactive tissue response.
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