Multi-wavelength light emitting diode-based disposable optrode array for in vivo optogenetic modulation
- 주제(키워드) implanted neurostimulators , neuroscience , optical fibers , optogenetics
- 주제(기타) Biochemical Research Methods; Biophysics; Optics
- 설명문(일반) [Jeon, Saeyeong; Lee, Hongkyun; Kim, Yong-Kweon] Seoul Natl Univ, Dept Elect & Comp Engn, Seoul, South Korea; [Kim, Jae-Hyun; Lee, Seung-Hee] Korea Adv Inst Sci & Technol, Dept Biol Sci, Daejeon, South Korea; [Kim, Yong-Kweon] Seoul Natl Univ, Inst Elect Power Res, Seoul, South Korea; [Jun, Sang Beom; Ji, Chang-Hyeon] Ewha Womans Univ, Dept Elect & Elect Engn, 52 Ewhayeodae Gil, Seoul 03760, South Korea
- 등재 SCIE, SCOPUS
- 발행기관 WILEY-V C H VERLAG GMBH
- 발행년도 2019
- URI http://www.dcollection.net/handler/ewha/000000160229
- 본문언어 영어
- Published As http://dx.doi.org/10.1002/jbio.201800343
- PubMed https://pubmed.ncbi.nlm.nih.gov/30588762
초록/요약
We present a light emitting diode (LED)-based optical waveguide array that can optogenetically modulate genetically targeted neurons in the brain. The reusable part of the system consists of control electronics and conventional multi-wavelength LED. The disposable part comprises optical fibers assembled with microlens array fabricated on a silicon die. Both parts can be easily assembled and separated by snap fit structure. Measured light intensity is 3.35 mW/mm(2) at 469 nm and 0.29 mW/mm(2) at 590 nm when the applied current is 80 mA. In all the tested conditions, the light-induced temperature rise is under 0.5 degrees C and over 90% of the relative light intensity is maintained at 2 mm-distance from the fiber tips. We further tested the efficiency of the optical array in vivo at 469 nm. When the optical array delivers light stimulation on to the visual cortex of a mouse expressing channelrhodopsin-2, the neural activity is significantly increased. The light-driven neural activity is successfully transformed into a percept of the mouse, showing significant learning of the task detecting the cortical stimulation. Our results demonstrate that the proposed optical array interfaces well with the neural circuits in vivo and the system is applicable to guide animal behaviors.
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